Biotechnology

Therapies Targeting Brain Diseases: Genomic and Lipidomic Approaches

Gene Therapy

Lipid Therapy

Functionalized Nanosystems

Targeted Nuclei Acid Delivery

Brain Cancer


Research lines

Development of a targeted therapeutic approach towards glioblastoma pathogenesis: modulation of the activity of key miRNAs combined with new generation anti-angiogenic chemotherapy

A membrane lipid therapy-based approach for glioblastoma and other brain diseases: modulation of membrane lipid composition by addressing key-enzymes of lipid metabolism using RNA interference technology

Development of immunotherapeutic strategies for glioblastoma: Evaluation of their efficacy in immunocompetent human cerebral organoid models

Overview

Having as a central mission to generate innovative gene and lipid therapies aimed at fighting brain diseases, namely brain cancer, the research developed in our group has been guided by the following objectives:

  • Identification of new molecular targets for the treatment of brain diseases, namely at the transcriptome and lipidome level in the ill cells;
  • Evaluation of the therapeutic efficacy of the developed gene therapy approaches and study of the molecular and cellular mechanisms associated to the therapeutic response, in cell and animal models of disease;
  • Study of the role of glioblastoma (GBM) stem-like cells in tumor progression and identification of new molecular targets involved in the renewal and differentiation of tumor stem-like cells;
  • Study of the communication between tumor and immune cells and identification of therapeutic targets involved in the tumor-specific immune environment. 


In parallel, in order to guarantee efficiency in gene therapy interventions, our group has also been enrolled in more fundamental research, with the following objectives:

  • Development of novel nanosystems based on lipids, surfactants, peptides and polymers, as technological platforms for efficient and specific delivery of different nucleic acids in target cells/tissues;
  • Physico-chemical characterization of the nanosystems and mechanistic studies of their interaction with target-cells, including cell internalization and intracellular traffic;
  • Application of the developed nanosystems in targeted gene therapy approaches using animal models of brain diseases, including GBM, towards potential clinical translation. 

Publications

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Information about journal articles, updated at 21-11-2021, from platform CIÊNCIAVITAE.

MiR-200c-based metabolic modulation in glioblastoma cells as a strategy to overcome tumor chemoresistance

Cardoso, Ana M; Morais, Catarina M; Sousa, Madalena; Rebelo, Olinda; Tão, Hermínio; Barbosa, Marcos; Pedroso de Lima, Maria C; Jurado, Amália S, 2021. Human Molecular Genetics. 2021. http://dx.doi.org/10.1093/hmg/ddab193 . 10.1093/hmg/ddab193 . Human Molecular Genetics

Improving pollutants environmental risk assessment using a multi model toxicity determination with in vitro, bacterial, animal and plant model systems: The case of the herbicide alachlor

Pereira, S.P.; Santos, S.M.A.; Fernandes, M.A.S.; Deus, C.M.; Martins, J.D.; Pedroso de Lima, M.C.; Vicente, J.A.F.; Videira, R.A.; Jurado, A.S., 2021. Environmental Pollution. 286. 2021. http://www.scopus.com/inward/record.url?eid=2-s2.0-85105603093&partnerID=MN8TOARS . 10.1016/j.envpol.2021.117239 . Environmental Pollution

Downregulation of long non-protein coding RNA MVIH impairs glioblastoma cell proliferation and invasion through an miR-302a-dependent mechanism

Cardoso, Ana M; Morais, Catarina M; Rebelo, Olinda; Tão, Hermínio; Barbosa, Marcos; Pedroso de Lima, Maria C; Jurado, Amália S, 2021. Human Molecular Genetics. 46 - 64. 1. 30. 2021. http://dx.doi.org/10.1093/hmg/ddab009 . 10.1093/hmg/ddab009 . Human Molecular Genetics

Differentiation of glioblastoma stem cells promoted by miR-128 or miR-302a overexpression enhances senescence-associated cytotoxicity of axitinib

Ana Cardoso, 2021. Human Molecular Genetics . 160 - 171. 30. 2021. doi: 10.1093/hmg/ddab011. . Human Molecular Genetics

Lauroylated Histidine-Enriched S413-PV Peptide as an Efficient Gene Silencing Mediator in Cancer Cells

Morais, Catarina M.; Cardoso, Ana M.; Aguiar, Luísa; Vale, Nuno; Nóbrega, Clévio; Zuzarte, Mónica; Gomes, Paula; Pedroso de Lima, Maria C.; Jurado, Amália S., 2020. Pharmaceutical Research. 10. 37. 2020. http://dx.doi.org/10.1007/s11095-020-02904-x . 10.1007/s11095-020-02904-x . Pharmaceutical Research

Physicochemical characterization and targeting performance of triphenylphosphonium nano-polyplexes

Faria, Rúben; Albuquerque, Tânia; Neves, Ana R.; Bhatt, Himanshu; Biswas, Swati; Cardoso, Ana M.; Pedroso de Lima, Maria C.; Jurado, Amália S.; Costa, Diana, 2020. Journal of Molecular Liquids. 316. 2020. http://dx.doi.org/10.1016/j.molliq.2020.113873 . 10.1016/j.molliq.2020.113873 . Journal of Molecular Liquids

Lysosomal Storage Disease-Associated Neuropathy: Targeting Stable Nucleic Acid Lipid Particle (SNALP)-Formulated siRNAs to the Brain as a Therapeutic Approach

Coutinho, Maria Francisca; Santos, Juliana Inês; S. Mendonça, Liliana; Matos, Liliana; Prata, Maria João; S. Jurado, Amália; Pedroso de Lima, Maria C.; Alves, Sandra, 2020. International Journal of Molecular Sciences. 16. 21. 2020. http://dx.doi.org/10.3390/ijms21165732 . 10.3390/ijms21165732 . International Journal of Molecular Sciences

Glucosylceramide synthase silencing combined with the receptor tyrosine kinase inhibitor axitinib as a new multimodal strategy for glioblastoma

Morais, C.M.; Cunha, P.P.; Melo, T.; Cardoso, A.M.; Domingues, P.; Domingues, M.R.; Pedroso de Lima, M.C.; Jurado, A.S., 2019. Human Molecular Genetics. 3664 - 3679. 21. 28. 2019. http://www.scopus.com/inward/record.url?eid=2-s2.0-85077223998&partnerID=MN8TOARS . 10.1093/hmg/ddz152 . Human Molecular Genetics

Acylation of the S4<inf>13</inf>-PV cell-penetrating peptide as a means of enhancing its capacity to mediate nucleic acid delivery: Relevance of peptide/lipid interactions

Morais, C.M.; Cardoso, A.M.; Cunha, P.P.; Aguiar, L.; Vale, N.; Lage, E.; Pinheiro, M.; et al, 2018. Biochimica et Biophysica Acta - Biomembranes. 2619 - 2634. 12. 1860. 2018. http://www.scopus.com/inward/record.url?eid=2-s2.0-85054439165&partnerID=MN8TOARS . 10.1016/j.bbamem.2018.10.002 . Biochimica et Biophysica Acta - Biomembranes

Multimodal highly fluorescent-magnetic nanoplatform to target transferrin receptors in cancer cells

Cabral Filho, P.E.; Cabrera, M.P.; Cardoso, A.L.C.; Santana, O.A.; Geraldes, C.F.G.C.; Santos, B.S.; Pedroso de Lima, M.C.; Pereira, G.A.L.; Fontes, A., 2018. Biochimica et Biophysica Acta - General Subjects. 2788 - 2796. 12. 1862. 2018. http://www.scopus.com/inward/record.url?eid=2-s2.0-85052949793&partnerID=MN8TOARS . 10.1016/j.bbagen.2018.08.014 . Biochimica et Biophysica Acta - General Subjects

Mechanistic study of the adjuvant effect of chitosan-aluminum nanoparticles

Lebre, F.; Pedroso de Lima, M.C.; Lavelle, E.C.; Borges, O., 2018. International Journal of Pharmaceutics. 7 - 15. 1-2. 552. 2018. http://www.scopus.com/inward/record.url?eid=2-s2.0-85053833190&partnerID=MN8TOARS . 10.1016/j.ijpharm.2018.09.044 . International Journal of Pharmaceutics

High-throughput screening uncovers miRNAs enhancing glioblastoma cell susceptibility to tyrosine kinase inhibitors

Pedro P Cunha; Pedro M Costa; Catarina M Morais; Inês R Lopes; Ana M Cardoso; Ana L Cardoso; Miguel Mano; Amália S Jurado; Maria C Pedroso de Lima, 2017. Human Molecular Genetics. 4375 - 4387. 22. 26. 2017. https://doi.org/10.1093/hmg/ddx323 . 10.1093/hmg/ddx323 . Human Molecular Genetics

Resveratrol stimulates the metabolic reprogramming of human CD4+T cells to enhance effector function

Marco Craveiro; Gaspard Cretenet; Cédric Mongellaz; Maria I. Matias; Olivier Caron; Maria C. Pedroso de Lima; Valérie S. Zimmermann; et al, 2017. Science Signaling. eaal3024 - eaal3024. 501. 10. 2017. https://doi.org/10.1126/scisignal.aal3024 . 10.1126/scisignal.aal3024 . Science Signaling

Association of chitosan and aluminium as a new adjuvant strategy for improved vaccination

Lebre, F.; Bento, D.; Ribeiro, J.; Colaço, M.; Borchard, G.; Pedroso De Lima, M.C.P.; Borges, O., 2017. International Journal of Pharmaceutics. 103 - 114. 1-2. 527. 2017. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019758926&partnerID=MN8TOARS . 10.1016/j.ijpharm.2017.05.028 . International Journal of Pharmaceutics

MicroRNA deregulation and chemotaxis and phagocytosis impairment in Alzheimer's disease

Guedes, J.R.; Santana, I.; Cunha, C.; Duro, D.; Almeida, M.R.; Cardoso, A.M.; de Lima, M.C.P.; Cardoso, A.L., 2016. Alzheimer's and Dementia: Diagnosis, Assessment and Disease Monitoring. 7 - 17. 3. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956620147&partnerID=MN8TOARS . 10.1016/j.dadm.2015.11.004 . Alzheimer's and Dementia: Diagnosis, Assessment and Disease Monitoring

Enhancing glioblastoma cell sensitivity to chemotherapeutics: A strategy involving survivin gene silencing mediated by gemini surfactant-based complexes

Cruz, R.Q.; Morais, C.M.; Cardoso, A.M.; Silva, S.G.; Vale, M.L.; Marques, E.F.; Pedroso De Lima, M.C.; Jurado, A.S., 2016. European Journal of Pharmaceutics and Biopharmaceutics. 7 - 18. 104. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84964330230&partnerID=MN8TOARS . 10.1016/j.ejpb.2016.04.014 . European Journal of Pharmaceutics and Biopharmaceutics

Intranasal Administration of Novel Chitosan Nanoparticle/DNA Complexes Induces Antibody Response to Hepatitis B Surface Antigen in Mice

Lebre, F.; Borchard, G.; Faneca, H.; Pedroso De Lima, M.C.; Borges, O., 2016. Molecular Pharmaceutics. 472 - 482. 2. 13. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84957308940&partnerID=MN8TOARS . 10.1021/acs.molpharmaceut.5b00707 . Molecular Pharmaceutics

Safety profile of the intravenous administration of brain-targeted stable nucleic acid lipid particles

Conceição, M.; Mendonça, L.; Nóbrega, C.; Gomes, C.; Costa, P.; Hirai, H.; Moreira, J.N.; et al, 2016. Data in Brief. 700 - 705. 6. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956617357&partnerID=MN8TOARS . 10.1016/j.dib.2016.01.017 . Data in Brief

Role of microRNAs in the regulation of innate immune cells under neuroinflammatory conditions

Cardoso, A.L.; Guedes, J.R.; De Lima, M.C.P., 2016. Current Opinion in Pharmacology. 1 - 9. 26. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84942921906&partnerID=MN8TOARS . 10.1016/j.coph.2015.09.001 . Current Opinion in Pharmacology

CdTe quantum dots as fluorescent probes to study transferrin receptors in glioblastoma cells

Cabral Filho, P.E.; Cardoso, A.L.C.; Pereira, M.I.A.; Ramos, A.P.M.; Hallwas, F.; Castro, M.M.C.A.; Geraldes, C.F.G.C.; et al, 2016. Biochimica et Biophysica Acta - General Subjects. 28 - 35. 1. 1860. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84946430431&partnerID=MN8TOARS . 10.1016/j.bbagen.2015.09.021 . Biochimica et Biophysica Acta - General Subjects

Intravenous administration of brain-targeted stable nucleic acid lipid particles alleviates Machado-Joseph disease neurological phenotype

Conceição, M.; Mendonça, L.; Nóbrega, C.; Gomes, C.; Costa, P.; Hirai, H.; Moreira, J.N.; et al, 2016. Biomaterials. 124 - 137. 82. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956644943&partnerID=MN8TOARS . 10.1016/j.biomaterials.2015.12.021 . Biomaterials

MicroRNAs in glioblastoma: Role in pathogenesis and opportunities for targeted therapies

Costa, P.M.; Cardoso, A.L.; Mano, M.; de Lima, M.C.P., 2015. CNS and Neurological Disorders - Drug Targets. 222 - 238. 2. 14. 2015. http://www.scopus.com/inward/record.url?eid=2-s2.0-84930907378&partnerID=MN8TOARS . 10.2174/1871527314666150116123610 . CNS and Neurological Disorders - Drug Targets

Gemini Surfactants Mediate Efficient Mitochondrial Gene Delivery and Expression

Cardoso, A.M.; Morais, C.M.; Cruz, A.R.; Cardoso, A.L.; Silva, S.G.; Do Vale, M.L.; Marques, E.F.; Pedroso De Lima, M.C.; Jurado, A.S., 2015. Molecular Pharmaceutics. 716 - 730. 3. 12. 2015. http://www.scopus.com/inward/record.url?eid=2-s2.0-84924023696&partnerID=MN8TOARS . 10.1021/mp5005349 . Molecular Pharmaceutics

New serine-derived gemini surfactants as gene delivery systems

Cardoso, A.M.; Morais, C.M.; Cruz, A.R.; Silva, S.G.; Do Vale, M.L.; Marques, E.F.; De Lima, M.C.P.; Jurado, A.S., 2015. European Journal of Pharmaceutics and Biopharmaceutics. 347 - 356. 89. 2015. http://www.scopus.com/inward/record.url?eid=2-s2.0-84920830778&partnerID=MN8TOARS . 10.1016/j.ejpb.2014.12.013 . European Journal of Pharmaceutics and Biopharmaceutics

MiRNA-21 silencing mediated by tumor-targeted nanoparticles combined with sunitinib: A new multimodal gene therapy approach for glioblastoma

Costa, P.M.; Cardoso, A.L.; Custódia, C.; Cunha, P.; Pereira De Almeida, L.; Pedroso De Lima, M.C., 2015. Journal of Controlled Release. 31 - 39. 207. 2015. http://www.scopus.com/inward/record.url?eid=2-s2.0-84926665383&partnerID=MN8TOARS . 10.1016/j.jconrel.2015.04.002 . Journal of Controlled Release

Increased gene delivery efficiency and specificity of a lipid-based nanosystem incorporating a glycolipid

Magalhães, M.; Farinha, D.; de Lima, M.C.P.; Faneca, H., 2014. International Journal of Nanomedicine. 4979 - 4989. 9. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84930260157&partnerID=MN8TOARS . 10.2147/IJN.S69822 . International Journal of Nanomedicine

MicroRNA modulation combined with sunitinib as a novel therapeutic strategy for pancreatic cancer

Passadouro, M.; Pedroso de Lima, M.C.; Faneca, H., 2014. International Journal of Nanomedicine. 3203 - 3217. 1. 9. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84903878322&partnerID=MN8TOARS . 10.2147/IJN.S64456 . International Journal of Nanomedicine

Specific and efficient gene delivery mediated by an asialofetuin-associated nanosystem

Farinha, D.; Pedroso De Lima, M.C.; Faneca, H., 2014. International Journal of Pharmaceutics. 366 - 374. 1-2. 473. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904968165&partnerID=MN8TOARS . 10.1016/j.ijpharm.2014.07.019 . International Journal of Pharmaceutics

Early miR-155 upregulation contributes to neuroinflammation in Alzheimer's disease triple transgenic mouse model.

Maria C. Pedroso de Lima, 2014. Human Molecular Genetics. 2014. http://www.ncbi.nlm.nih.gov/pubmed/24990149 . 10.1093/hmg/ddu348 . Human Molecular Genetics

MicroRNA modulation combined with sunitinib as a novel therapeutic strategy for pancreatic cancer

Henrique Faneca; Marta Passadouro; Maria Pedroso de Lima, 2014. International Journal of Nanomedicine. 3203 - 3203. 2014. https://doi.org/10.2147%2Fijn.s64456 . 10.2147/ijn.s64456 . International Journal of Nanomedicine

Application of thermoresponsive PNIPAAM-b-PAMPTMA diblock copolymers in siRNA delivery

Cardoso, A.M.; Calejo, M.T.; Morais, C.M.; Cardoso, A.L.; Cruz, R.; Zhu, K.; Pedroso De Lima, M.C.; Jurado, A.S.; Nyström, B., 2014. Molecular Pharmaceutics. 819 - 827. 3. 11. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84901742907&partnerID=MN8TOARS . 10.1021/mp400510a . Molecular Pharmaceutics

Bis-quaternary gemini surfactants as components of nonviral gene delivery systems: A comprehensive study from physicochemical properties to membrane interactions

Cardoso, A.M.; Morais, C.M.; Silva, S.G.; Marques, E.F.; De Lima, M.C.P.; Jurado, M.A.S., 2014. International Journal of Pharmaceutics. 57 - 69. 1-2. 474. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84906267891&partnerID=MN8TOARS . 10.1016/j.ijpharm.2014.08.011 . International Journal of Pharmaceutics

Sustained release of naltrexone from Poly(N-Isopropylacrylamide) microgels

Kjøniksen, A.-L.; Calejo, M.T.; Zhu, K.; Cardoso, A.M.S.; De Lima, M.C.P.; Jurado, A.S.; Nyström, B.; Sande, S.A., 2014. Journal of Pharmaceutical Sciences. 227 - 234. 1. 103. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84890551451&partnerID=MN8TOARS . 10.1002/jps.23780 . Journal of Pharmaceutical Sciences

Involvement of MicroRNA in microglia-mediated immune response

Guedes, J.; Cardoso, A.L.C.; Pedroso De Lima, M.C., 2013. Clinical and Developmental Immunology. 2013. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878648745&partnerID=MN8TOARS . 10.1155/2013/186872 . Clinical and Developmental Immunology

Impact of anti-PLK1 siRNA-containing F3-targeted liposomes on the viability of both cancer and endothelial cells

Gomes-Da-Silva, L.C.; Ramalho, J.S.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N., 2013. European Journal of Pharmaceutics and Biopharmaceutics. 356 - 364. 3 PART A. 85. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84888308868&partnerID=MN8TOARS . 10.1016/j.ejpb.2013.04.007 . European Journal of Pharmaceutics and Biopharmaceutics

Tumor-targeted chlorotoxin-coupled nanoparticles for nucleic acid delivery to glioblastoma cells: A promising system for glioblastoma treatment

Costa, P.M.; Cardoso, A.L.; Mendonça, L.S.; Serani, A.; Custódia, C.; Conceição, M.; Simões, S.; et al, 2013. Molecular Therapy - Nucleic Acids. 2. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879940866&partnerID=MN8TOARS . 10.1038/mtna.2013.30 . Molecular Therapy - Nucleic Acids

Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform

Gomes-Da-Silva, L.C.; Fernández, Y.; Abasolo, I.; Schwartz, S.; Ramalho, J.S.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N., 2013. Nanomedicine. 1397 - 1413. 9. 8. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84893195240&partnerID=MN8TOARS . 10.2217/nnm.12.174 . Nanomedicine

Comparison of the efficiency of complexes based on S4<inf>13</inf>-PV cell-penetrating peptides in plasmid DNA and siRNA delivery

Cardoso, A.M.; Trabulo, S.; Cardoso, A.L.; Maia, S.; Gomes, P.; Jurado, A.S.; Pedroso De Lima, M.C., 2013. Molecular Pharmaceutics. 2653 - 2666. 7. 10. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879735661&partnerID=MN8TOARS . 10.1021/mp400078h . Molecular Pharmaceutics

Synthesis of gemini surfactants and evaluation of their interfacial and cytotoxic properties: Exploring the multifunctionality of serine as headgroup

Silva, S.G.; Alves, C.; Cardoso, A.M.S.; Jurado, A.S.; Pedroso De Lima, M.C.; Vale, M.L.C.; Marques, E.F., 2013. European Journal of Organic Chemistry. 1758 - 1769. 9. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875008251&partnerID=MN8TOARS . 10.1002/ejoc.201201396 . European Journal of Organic Chemistry

Cell-penetrating peptides as nucleic acid delivery systems: From biophysics to biological applications

Trabulo, S.; Cardoso, A.L.; Cardoso, A.M.S.; Morais, C.M.; Jurado, A.S.; Pedroso de Lima, M.C., 2013. Current Pharmaceutical Design. 2895 - 2923. 16. 19. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878655380&partnerID=MN8TOARS . 10.2174/1381612811319160006 . Current Pharmaceutical Design

Impact of PLK-1 silencing on endothelial cells and cancer cells of diverse histological origin

Gomes, C.P.; Gomes-da-Silva, L.C.; Ramalho, J.S.; de Lima, M.C.P.; Simões, S.; Moreira, J.N., 2013. Current Gene Therapy. 189 - 201. 3. 13. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878633310&partnerID=MN8TOARS . 10.2174/1566523211313030004 . Current Gene Therapy

Preventive cancer stem cell-based vaccination reduces liver metastasis development in a rat colon carcinoma syngeneic model

Duarte, S.; Momier, D.; Baqué, P.; Casanova, V.; Loubat, A.; Samson, M.; Guigonis, J.-M.; et al, 2013. Stem Cells. 423 - 432. 3. 31. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874818661&partnerID=MN8TOARS . 10.1002/stem.1292 . Stem Cells

In vitro cytotoxicity of a thermoresponsive gel system combining ethyl(hydroxyethyl) cellulose and lysine-based surfactants

Calejo, M.T.; Cardoso, A.M.S.; Marques, E.F.; Araújo, M.J.; Kjøniksen, A.-L.; Sande, S.A.; de Lima, M.C.P.; Jurado, A.S.; Nyström, B., 2013. Colloids and Surfaces B: Biointerfaces. 682 - 686. 102. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84867835593&partnerID=MN8TOARS . 10.1016/j.colsurfb.2012.09.033 . Colloids and Surfaces B: Biointerfaces

Temperature-responsive cationic block copolymers as nanocarriers for gene delivery

Calejo, M.T.; Cardoso, A.M.S.; Kjøniksen, A.-L.; Zhu, K.; Morais, C.M.; Sande, S.A.; Cardoso, A.L.; et al, 2013. International Journal of Pharmaceutics. 105 - 114. 1. 448. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875930565&partnerID=MN8TOARS . 10.1016/j.ijpharm.2013.03.028 . International Journal of Pharmaceutics

MicroRNA-21 silencing enhances the cytotoxic effect of the antiangiogenic drug sunitinib in glioblastoma

Costa, P.M.; Cardoso, A.L.; Nóbrega, C.; Pereira de almeida, L.F.; Bruce, J.N.; Canoll, P.; Pedroso de Lima, M.C., 2013. Human Molecular Genetics. 904 - 918. 5. 22. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873438180&partnerID=MN8TOARS . 10.1093/hmg/dds496 . Human Molecular Genetics

Micrornas as molecular targets for cancer therapy: On the modulation of microrna expression

Costa, P.M.; De Lima, M.C.P., 2013. Pharmaceuticals. 1195 - 1220. 10. 6. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84885029680&partnerID=MN8TOARS . 10.3390/ph6101195 . Pharmaceuticals

Toward a siRNA-containing nanoparticle targeted to breast cancer cells and the tumor microenvironment

Gomes-Da-Silva, L.C.; Santos, A.O.; Bimbo, L.M.; Moura, V.; Ramalho, J.S.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N., 2012. International Journal of Pharmaceutics. 9 - 19. 1-2. 434. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864129727&partnerID=MN8TOARS . 10.1016/j.ijpharm.2012.05.018 . International Journal of Pharmaceutics

Thermoresponsive hydrogels with low toxicity from mixtures of ethyl(hydroxyethyl) cellulose and arginine-based surfactants

Calejo, M.T.; Kjoniksen, A.-L.; Pinazo, A.; Pérez, L.; Cardoso, A.M.S.; Pedroso De Lima, M.C.; Jurado, A.S.; Sande, S.A.; Nyström, B., 2012. International Journal of Pharmaceutics. 454 - 462. 1-2. 436. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84865288628&partnerID=MN8TOARS . 10.1016/j.ijpharm.2012.07.018 . International Journal of Pharmaceutics

S4(13)-PV cell-penetrating peptide induces physical and morphological changes in membrane-mimetic lipid systems and cell membranes: Implications for cell internalization

Cardoso, A.M.S.; Trabulo, S.; Cardoso, A.L.; Lorents, A.; Morais, C.M.; Gomes, P.; Nunes, C.; et al, 2012. Biochimica et Biophysica Acta - Biomembranes. 877 - 888. 3. 1818. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84856059612&partnerID=MN8TOARS . 10.1016/j.bbamem.2011.12.022 . Biochimica et Biophysica Acta - Biomembranes

Lipid-based nanoparticles for siRNA delivery in cancer therapy: Paradigms and challenges

Gomes-Da-Silva, L.C.; Fonseca, N.A.; Moura, V.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N., 2012. Accounts of Chemical Research. 1163 - 1171. 7. 45. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863922166&partnerID=MN8TOARS . 10.1021/ar300048p . Accounts of Chemical Research

Targeted lipid-based systems for siRNA delivery

Mendonça, L.S.; Pedroso De Lima, M.C.; Simöes, S., 2012. Journal of Drug Delivery Science and Technology. 65 - 73. 1. 22. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84857427765&partnerID=MN8TOARS . Journal of Drug Delivery Science and Technology

Isolation of head and neck squamous carcinoma cancer stem-like cells in a syngeneic mouse model and analysis of hypoxia effect

Duarte, S.; Loubat, A.; Momier, D.; Topi, M.; Faneca, H.; Pedroso De Lima, M.C.; Carle, G.F.; Pierrefite-Carle, V., 2012. Oncology Reports. 1057 - 1062. 3. 28. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84865312688&partnerID=MN8TOARS . 10.3892/or.2012.1904 . Oncology Reports

Suicide gene therapy in cancer: Where do we stand now?

Duarte, S.; Carle, G.; Faneca, H.; Lima, M.C.P.D.; Pierrefite-Carle, V., 2012. Cancer Letters. 160 - 170. 2. 324. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864392111&partnerID=MN8TOARS . 10.1016/j.canlet.2012.05.023 . Cancer Letters

Targeted and intracellular triggered delivery of therapeutics to cancer cells and the tumor microenvironment: Impact on the treatment of breast cancer

Moura, V.; Lacerda, M.; Figueiredo, P.; Corvo, M.L.; Cruz, M.E.M.; Soares, R.; De Lima, M.C.P.; Simões, S.; Moreira, J.N., 2012. Breast Cancer Research and Treatment. 61 - 73. 1. 133. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84860334520&partnerID=MN8TOARS . 10.1007/s10549-011-1688-7 . Breast Cancer Research and Treatment

miR-155 modulates microglia-mediated immune response by down-regulating SOCS-1 and promoting cytokine and nitric oxide production

Cardoso, A.L.; Guedes, J.R.; Pereira de Almeida, L.; Pedroso de Lima, M.C., 2012. Immunology. 73 - 88. 1. 135. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-83055176904&partnerID=MN8TOARS . 10.1111/j.1365-2567.2011.03514.x . Immunology

Folate-associated lipoplexes mediate efficient gene delivery and potent antitumoral activity in vitro and in vivo

Duarte, S.; Faneca, H.; Lima, M.C.P.D., 2012. International Journal of Pharmaceutics. 365 - 377. 2. 423. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84856552664&partnerID=MN8TOARS . 10.1016/j.ijpharm.2011.12.035 . International Journal of Pharmaceutics

PDGF-B-mediated downregulation of miR-21: New insights into PDGF signaling in glioblastoma

Costa, P.M.; Cardoso, A.L.; Pereira de almeida, L.F.; Bruce, J.N.; Canoll, P.; Pedroso de lima, M.C., 2012. Human Molecular Genetics. 5118 - 5130. 23. 21. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84869025536&partnerID=MN8TOARS . 10.1093/hmg/dds358 . Human Molecular Genetics

Survivin silencing as a promising strategy to enhance the sensitivity of cancer cells to chemotherapeutic agents

Trabulo, S.; Cardoso, A.M.; Santos-Ferreira, T.; Cardoso, A.L.; Simões, S.; Pedroso De Lima, M.C., 2011. Molecular Pharmaceutics. 1120 - 1131. 4. 8. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79961082146&partnerID=MN8TOARS . 10.1021/mp100426e . Molecular Pharmaceutics

Inclusion of a single-tail amino acid-based amphiphile in a lipoplex formulation: Effects on transfection efficiency and physicochemical properties

Lundberg, D.; Faneca, H.; MorÁn, M.D.C.; Pedroso De Lima, M.C.; Miguel, M.D.G.; Lindman, B., 2011. Molecular Membrane Biology. 42 - 53. 1. 28. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-78651279670&partnerID=MN8TOARS . 10.3109/09687688.2010.508879 . Molecular Membrane Biology

Gemini surfactant dimethylene-1,2-bis(tetradecyldimethylammonium bromide)-based gene vectors: A biophysical approach to transfection efficiency

Cardoso, A.M.S.; Faneca, H.; Almeida, J.A.S.; Pais, A.A.C.C.; Marques, E.F.; De Lima, M.C.P.; Jurado, A.S., 2011. Biochimica et Biophysica Acta - Biomembranes. 341 - 351. 1. 1808. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-78649777308&partnerID=MN8TOARS . 10.1016/j.bbamem.2010.09.026 . Biochimica et Biophysica Acta - Biomembranes

Progress towards a needle-free hepatitis B vaccine

Lebre, F.; Borchard, G.; De Lima, M.C.P.; Borges, O., 2011. Pharmaceutical Research. 986 - 1012. 5. 28. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955607675&partnerID=MN8TOARS . 10.1007/s11095-010-0314-4 . Pharmaceutical Research

In situ forming chitosan hydrogels prepared via ionic/covalent Co-cross-linking

Moura, M.J.; Faneca, H.; Lima, M.P.; Gil, M.H.; Figueiredo, M.M., 2011. Biomacromolecules. 3275 - 3284. 9. 12. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052771947&partnerID=MN8TOARS . 10.1021/bm200731x . Biomacromolecules

Non-covalent association of folate to lipoplexes: A promising strategy to improve gene delivery in the presence of serum

Duarte, S.; Faneca, H.; Pedroso De Lima, M.C., 2011. Journal of Controlled Release. 264 - 272. 3. 149. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79951941675&partnerID=MN8TOARS . 10.1016/j.jconrel.2010.10.032 . Journal of Controlled Release

In vitro modulation of Bcl-2 levels in small cell lung cancer cells: Effects on cell viability

Santos, A.O.; Pereira, J.P.; Pedroso de Lima, M.C.; Simões, S.; Moreira, J.N., 2010. Brazilian Journal of Medical and Biological Research. 1001 - 1009. 10. 43. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-78149263712&partnerID=MN8TOARS . 10.1590/S0100-879X2010007500099 . Brazilian Journal of Medical and Biological Research

S4<inf>13</inf>-PV cell-penetrating peptide forms nanoparticle-like structures to gain entry into cells

Padari, K.; Koppel, K.; Lorents, A.; Hällbrink, M.; Mano, M.; Pedroso De Lima, M.C.; Pooga, M., 2010. Bioconjugate Chemistry. 774 - 783. 4. 21. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-77951285705&partnerID=MN8TOARS . 10.1021/bc900577e . Bioconjugate Chemistry

Tf-lipoplex-mediated c-Jun silencing improves neuronal survival following excitotoxic damage in vivo

Cardoso, A.L.C.; Costa, P.; de Almeida, L.P.; Simões, S.; Plesnila, N.; Culmsee, C.; Wagner, E.; Pedroso de Lima, M.C., 2010. Journal of Controlled Release. 392 - 403. 3. 142. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-77349098528&partnerID=MN8TOARS . 10.1016/j.jconrel.2009.11.004 . Journal of Controlled Release

Silencing ataxin-3 mitigates degeneration in a rat model of Machado-Joseph disease: No role for wild-type ataxin-3?

Alves, S.; Nascimento-Ferreira, I.; Dufour, N.; Hassig, R.; Auregan, G.; Nóbrega, C.; Brouillet, E.; et al, 2010. Human Molecular Genetics. 2380 - 2394. 12. 19. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-77955290123&partnerID=MN8TOARS . 10.1093/hmg/ddq111 . Human Molecular Genetics

Physicochemical properties of transferrin-associated lipopolyplexes and their role in biological activity

Penacho, N.; Rosa, M.; Lindman, B.; Miguel, M.G.; Simões, S.; de Lima, M.C.P., 2010. Colloids and Surfaces B: Biointerfaces. 207 - 214. 1. 76. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-74149085531&partnerID=MN8TOARS . 10.1016/j.colsurfb.2009.10.034 . Colloids and Surfaces B: Biointerfaces

Design of peptide-targeted liposomes containing nucleic acids

Santos, A.O.; da Silva, L.C.G.; Bimbo, L.M.; de Lima, M.C.P.; Simões, S.; Moreira, J.N., 2010. Biochimica et Biophysica Acta - Biomembranes. 433 - 441. 3. 1798. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-76749124054&partnerID=MN8TOARS . 10.1016/j.bbamem.2009.12.001 . Biochimica et Biophysica Acta - Biomembranes

Transferrin receptor-targeted liposomes encapsulating anti-BCR-ABL siRNA or asodn for chronic myeloid leukemia treatment

Mendonça, L.S.; Firmino, F.; Moreira, J.N.; De Lima, M.C.P.; Simões, S., 2010. Bioconjugate Chemistry. 157 - 168. 1. 21. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-74949127407&partnerID=MN8TOARS . 10.1021/bc9004365 . Bioconjugate Chemistry

Co-encapsulation of anti-BCR-ABL siRNA and imatinib mesylate in transferrin receptor-targeted sterically stabilized liposomes for chronic myeloid leukemia treatment

Mendonça, L.S.; Moreira, J.N.; De Lima, M.C.P.; Simões, S., 2010. Biotechnology and Bioengineering. 884 - 893. 5. 107. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-77958590068&partnerID=MN8TOARS . 10.1002/bit.22858 . Biotechnology and Bioengineering

A non-covalent strategy combining cationic lipids and CPPs to enhance the delivery of splice correcting oligonucleotides

Trabulo, S.; Resina, S.; Simões, S.; Lebleu, B.; Pedroso de Lima, M.C., 2010. Journal of Controlled Release. 149 - 158. 2. 145. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-77953961849&partnerID=MN8TOARS . 10.1016/j.jconrel.2010.03.021 . Journal of Controlled Release

Methods to monitor liposome fusion, permeability, and interaction with cells.

Düzgünes, N.; Faneca, H.; Lima, M.C., 2010. Methods in molecular biology (Clifton, N.J.). 209 - 232. 606. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-77649218400&partnerID=MN8TOARS . 10.1007/978-1-60761-447-0_16 . Methods in molecular biology (Clifton, N.J.)

Interaction of proteinase inhibitors with phospholipid vesicles is modulated by pH

Silva-Lucca, R.A.; Faneca, H.M.S.; de Lima, M.C.P.; De Caroli, F.P.; Assis, M.L.; Sampaio, M.U.; Oliva, M.L.V., 2010. International Journal of Biological Macromolecules. 551 - 557. 4. 47. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-77956717001&partnerID=MN8TOARS . 10.1016/j.ijbiomac.2010.07.011 . International Journal of Biological Macromolecules

Cell-penetrating peptides-mechanisms of cellular uptake and generation of delivery systems

Trabulo, S.; Cardoso, A.L.; Mano, M.; de Lima, M.C.P., 2010. Pharmaceuticals. 961 - 993. 4. 3. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-77952146840&partnerID=MN8TOARS . 10.3390/ph3040961 . Pharmaceuticals

Polyethylenimine of various molecular weights as adjuvant for transfection mediated by cationic liposomes

Penacho, N.; Simões, S.; De Lima, M.P., 2009. Molecular Membrane Biology. 249 - 263. 4. 26. 2009. http://www.scopus.com/inward/record.url?eid=2-s2.0-67650308758&partnerID=MN8TOARS . 10.1080/09687680902766716 . Molecular Membrane Biology

Transferrin lipoplex-mediated suicide gene therapy of oral squamous cell carcinoma in an immunocompetent murine model and mechanisms involved in the antitumoral response

Neves, S.; Faneca, H.; Bertin, S.; Konopka, K.; Düzgünes, N.; Pierrefite-Carle, V.; Simões, S.; Pedroso De Lima, M.C., 2009. Cancer Gene Therapy. 91 - 101. 1. 16. 2009. http://www.scopus.com/inward/record.url?eid=2-s2.0-57649131464&partnerID=MN8TOARS . 10.1038/cgt.2008.60 . Cancer Gene Therapy

Vanadium compounds as therapeutic agents: Some chemical and biochemical studies

Faneca, H.; Figueiredo, V.A.; Tomaz, I.; Gonçalves, G.; Avecilla, F.; Pedroso de Lima, M.C.; Geraldes, C.F.G.C.; Pessoa, J.C.; Castro, M.M.C.A., 2009. Journal of Inorganic Biochemistry. 601 - 608. 4. 103. 2009. http://www.scopus.com/inward/record.url?eid=2-s2.0-62349102649&partnerID=MN8TOARS . 10.1016/j.jinorgbio.2008.11.004 . Journal of Inorganic Biochemistry

Simultaneous evaluation of viability and Bcl-2 in small-cell lung cancer

Santos, A.; Sarmento-Ribeiro, A.B.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N., 2008. Cytometry Part A. 1165 - 1172. 12. 73. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-56749165193&partnerID=MN8TOARS . 10.1002/cyto.a.20634 . Cytometry Part A

Synergistic antitumoral effect of vinblastine and HSV-Tk/GCV gene therapy mediated by albumin-associated cationic liposomes

Faneca, H.; Faustino, A.; Pedroso de Lima, M.C., 2008. Journal of Controlled Release. 175 - 184. 2. 126. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-38749101958&partnerID=MN8TOARS . 10.1016/j.jconrel.2007.12.005 . Journal of Controlled Release

Non-viral lipid-based nanoparticles for targeted cancer systemic gene silencing

Moreira, J.N.; Santos, A.; Moura, V.; De Uma, M.C.P.; Simões, S., 2008. Journal of Nanoscience and Nanotechnology. 2187 - 2204. 5. 8. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-47149083118&partnerID=MN8TOARS . 10.1166/jnn.2008.319 . Journal of Nanoscience and Nanotechnology

African swine fever virus p10 protein exhibits nuclear import capacity and accumulates in the nucleus during viral infection

Nunes-Correia, I.; Rodríguez, J.M.; Eulálio, A.; Carvalho, A.L.; Citovsky, V.; Simões, S.; Faro, C.; Salas, M.L.; Pedroso de Lima, M.C., 2008. Veterinary Microbiology. 47 - 59. 1-2. 130. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-46549086170&partnerID=MN8TOARS . 10.1016/j.vetmic.2007.12.010 . Veterinary Microbiology

Transferrin-associated lipoplexes as gene delivery systems: Relevance of mode of preparation and biophysical properties

Penacho, N.; Filipe, A.; Simões, S.; Pedroso De Lima, M.C., 2008. Journal of Membrane Biology. 141 - 152. 3. 221. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-40549096386&partnerID=MN8TOARS . 10.1007/s00232-008-9092-x . Journal of Membrane Biology

Allele-specific RNA silencing of mutant ataxin-3 mediates neuroprotection in a rat model of Machado-Joseph disease

Alves, S.; Nascimento-Ferreira, I.; Auregan, G.; Hassig, R.; Dufour, N.; Brouillet, E.; Pedroso de Lima, M.C.; et al, 2008. PLoS ONE. 10. 3. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-54449095083&partnerID=MN8TOARS . 10.1371/journal.pone.0003341 . PLoS ONE

S4<inf>13</inf>-PV cell penetrating peptide and cationic liposomes act synergistically to mediate intracellular delivery of plasmid DNA

Trabulo, S.; Mano, M.; Faneca, H.; Cardoso, A.L.; Duarte, S.; Henriques, A.; Paiva, A.; et al, 2008. Journal of Gene Medicine. 1210 - 1222. 11. 10. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149190435&partnerID=MN8TOARS . 10.1002/jgm.1247 . Journal of Gene Medicine

DNA pre-condensation with an amino acid-based cationic amphiphile. A viable approach for liposome-based gene delivery

Rosa, M.; Penacho, N.; Simões, S.; Lima, M.C.P.; Lindman, B.; Miguel, M.G., 2008. Molecular Membrane Biology. 23 - 34. 1. 25. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-37549013769&partnerID=MN8TOARS . 10.1080/09687680701499451 . Molecular Membrane Biology

Tf-lipoplexes for neuronal siRNA delivery: A promising system to mediate gene silencing in the CNS

Cardoso, A.L.C.; Simões, S.; de Almeida, L.P.; Plesnila, N.; Pedroso de Lima, M.C.; Wagner, E.; Culmsee, C., 2008. Journal of Controlled Release. 113 - 123. 2. 132. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-56349102126&partnerID=MN8TOARS . 10.1016/j.jconrel.2008.08.014 . Journal of Controlled Release

Striatal and nigral pathology in a lentiviral rat model of Machado-Joseph disease

Alves, S.; Régulier, E.; Nascimento-Ferreira, I.; Hassig, R.; Dufour, N.; Koeppen, A.; Carvalho, A.L.; et al, 2008. Human Molecular Genetics. 2071 - 2083. 14. 17. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-46249122383&partnerID=MN8TOARS . 10.1093/hmg/ddn106 . Human Molecular Genetics

Naked DNA and lipoplexes applications in cancer gene therapy,Applications de l'ADN nu ou associé àdes liposomes en thérapie génique anticancéreuse

Bertin, S.; Neves, S.; Pedroso De Lima, M.; Pierrefite-Carle, V., 2007. Bulletin du Cancer. 243 - 252. 3. 94. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-33947501342&partnerID=MN8TOARS . 10.1684/bdc.2007.0218 . Bulletin du Cancer

Evaluation of the antitumoral effect mediated by IL-12 and HSV-tk genes when delivered by a novel lipid-based system

Faneca, H.; Cabrita, A.S.; Simões, S.; Pedroso de Lima, M.C., 2007. Biochimica et Biophysica Acta - Biomembranes. 1093 - 1102. 5. 1768. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-34047252681&partnerID=MN8TOARS . 10.1016/j.bbamem.2006.12.017 . Biochimica et Biophysica Acta - Biomembranes

siRNA delivery by a transferrin-associated lipid-based vector: A non-viral strategy to mediate gene silencing

Cardoso, A.L.C.; Simões, S.; de Almeida, L.P.; Pelisek, J.; Culmsee, C.; Wagner, E.; Pedroso de Lima, M.C., 2007. Journal of Gene Medicine. 170 - 183. 3. 9. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-34047102911&partnerID=MN8TOARS . 10.1002/jgm.1006 . Journal of Gene Medicine

Cellular and molecular events associated with the antitumor response induced by the cytosine deaminase/5-fluorocytosine suicide gene therapy system in a rat liver metastasis model

Bertin, S.; Neves, S.; Gavelli, A.; Baqué, P.; Brossette, N.; Simões, S.; Pedroso De Lima, M.C.; Pierrefite-Carle, V., 2007. Cancer Gene Therapy. 858 - 866. 10. 14. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548606960&partnerID=MN8TOARS . 10.1038/sj.cgt.7701075 . Cancer Gene Therapy

African swine fever virus p37 structural protein is localized in nuclear foci containing the viral DNA at early post-infection times

Eulálio, A.; Nunes-Correia, I.; Salas, J.; Salas, M.L.; Simões, S.; Pedroso de Lima, M.C., 2007. Virus Research. 18 - 27. 1-2. 130. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-35748981502&partnerID=MN8TOARS . 10.1016/j.virusres.2007.05.009 . Virus Research

Interaction of S4<inf>13</inf>-PV cell penetrating peptide with model membranes: Relevance to peptide translocation across biological membranes

Mano, M.; Henriques, A.; Paiva, A.; Prieto, M.; Gavilanes, F.; Simões, S.; Pedroso de Lima, M.C., 2007. Journal of Peptide Science. 301 - 313. 5. 13. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-34248347468&partnerID=MN8TOARS . 10.1002/psc.842 . Journal of Peptide Science

Cellular uptake of S4<inf>13</inf>-PV peptide occurs upon conformational changes induced by peptide-membrane interactions

Mano, M.; Henriques, A.; Paiva, A.; Prieto, M.; Gavilanes, F.; Simões, S.; Pedroso de Lima, M.C., 2006. Biochimica et Biophysica Acta - Biomembranes. 336 - 346. 3. 1758. 2006. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646534241&partnerID=MN8TOARS . 10.1016/j.bbamem.2006.01.014 . Biochimica et Biophysica Acta - Biomembranes

Nuclear export of African swine fever virus p37 protein occurs through two distinct pathways and is mediated by three independent signals

Eulálio, A.; Nunes-Correia, I.; Carvalho, A.L.; Faro, C.; Citovsky, V.; Salas, J.; Salas, M.L.; Simões, S.; Pedroso De Lima, M.C., 2006. Journal of Virology. 1393 - 1404. 3. 80. 2006. http://www.scopus.com/inward/record.url?eid=2-s2.0-31144441675&partnerID=MN8TOARS . 10.1128/JVI.80.3.1393-1404.2006 . Journal of Virology

Transfection of oral cancer cells mediated by transferrin-associated lipoplexes: Mechanisms of cell death induced by herpes simplex virus thymidine kinase/ganciclovir therapy

Neves, S.S.; Sarmento-Ribeiro, A.B.; Simões, S.P.; Pedroso de Lima, M.C., 2006. Biochimica et Biophysica Acta - Biomembranes. 1703 - 1712. 11. 1758. 2006. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750502013&partnerID=MN8TOARS . 10.1016/j.bbamem.2006.08.021 . Biochimica et Biophysica Acta - Biomembranes

Targeting of sterically stabilised pH-sensitive liposomes to human T-leukaemia cells

Fonseca, C.; Moreira, J.N.; Ciudad, C.J.; Pedroso De Lima, M.C.; Simões, S., 2005. European Journal of Pharmaceutics and Biopharmaceutics. 359 - 366. 2. 59. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-12344294490&partnerID=MN8TOARS . 10.1016/j.ejpb.2004.08.012 . European Journal of Pharmaceutics and Biopharmaceutics

Cationic liposomes for gene delivery

Simões, S.; Filipe, A.; Faneca, H.; Mano, M.; Penacho, N.; Düzgünes, N.; de Lima, M.P., 2005. Expert Opinion on Drug Delivery. 237 - 254. 2. 2. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-23744433700&partnerID=MN8TOARS . 10.1517/17425247.2.2.237 . Expert Opinion on Drug Delivery

Liposomal and viral vectors for gene therapy of the central nervous system

Pedroso de Lima, M.C.; Girão da Cruz, M.T.; Cardoso, A.L.C.; Simões, S.; Pereira de Almeida, L., 2005. Current Drug Targets: CNS and Neurological Disorders. 453 - 465. 4. 4. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-23144438160&partnerID=MN8TOARS . 10.2174/1568007054546144 . Current Drug Targets: CNS and Neurological Disorders

Tf-lipoplex-mediated NGF gene transfer to the CNS: Neuronal protection and recovery in an excitotoxic model of brain injury

Girão da Cruz, M.T.; Cardoso, A.L.C.; de Almeida, L.P.; Simões, S.; Pedroso de Lima, M.C., 2005. Gene Therapy. 1242 - 1252. 16. 12. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-23944465946&partnerID=MN8TOARS . 10.1038/sj.gt.3302516 . Gene Therapy

On the mechanisms of the internalization of S4<inf>13</inf>-PV cell-penetrating peptide

Mano, M.; Teodósio, C.; Paiva, A.; Simões, S.; Pedroso De Lima, M.C., 2005. Biochemical Journal. 603 - 612. 2. 390. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-25144520907&partnerID=MN8TOARS . 10.1042/BJ20050577 . Biochemical Journal

The role of target membrane sialic acid residues in the fusion activity of the influenza virus: The effect of two types of ganglioside on the kinetics of membrane merging

Ramalho-Santos, J.; Pedroso De Lima, M.C., 2004. Cellular and Molecular Biology Letters. 337 - 351. 2. 9. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-3142546892&partnerID=MN8TOARS . Cellular and Molecular Biology Letters

Cytotoxic activity of metal complexes of biogenic polyamines: Polynuclear platinum(II) chelates

Teixeira, L.J.; Seabra, M.; Reis, E.; Da Cruz, M.T.G.; De Lima, M.C.P.; Pereira, E.; Miranda, M.A.; Marques, M.P.M., 2004. Journal of Medicinal Chemistry. 2917 - 2925. 11. 47. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-2542477134&partnerID=MN8TOARS . 10.1021/jm0311238 . Journal of Medicinal Chemistry

Association of albumin or protamine to lipoplexes: Enhancement of transfection and resistance to serum

Faneca, H.; Simões, S.; Pedroso de Lima, M.C., 2004. Journal of Gene Medicine. 681 - 692. 6. 6. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-13644260769&partnerID=MN8TOARS . 10.1002/jgm.550 . Journal of Gene Medicine

Caveolae as an additional route for influenza virus endocytosis in MDCK cells

Nunes-Correia, I.; Eulálio, A.; Nir, S.; Pedroso De Lima, M.C., 2004. Cellular and Molecular Biology Letters. 47 - 60. 1. 9. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-2342439156&partnerID=MN8TOARS . Cellular and Molecular Biology Letters

Voltammetric behaviour of oligonucleotide lipoplexes adsorbed onto glassy carbon electrodes

Piedade, J.A.P.; Mano, M.; Pedroso de Lima, M.C.; Oretskaya, T.S.; Oliveira-Brett, A.M., 2004. Journal of Electroanalytical Chemistry. 25 - 34. 1-2. 564. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-1342285697&partnerID=MN8TOARS . 10.1016/j.jelechem.2003.10.008 . Journal of Electroanalytical Chemistry

Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces

Piedade, J.A.P.; Mano, M.; Pedroso De Lima, M.C.; Oretskaya, T.S.; Oliveira-Brett, A.M., 2004. Biosensors and Bioelectronics. 975 - 984. 5. 20. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-7644240649&partnerID=MN8TOARS . 10.1016/j.bios.2004.06.014 . Biosensors and Bioelectronics

On the formulation of pH-sensitive liposomes with long circulation times

Simões, S.; Nuno Moreira, J.; Fonseca, C.; Düzgünes, N.; Pedroso De Lima, M.C., 2004. Advanced Drug Delivery Reviews. 947 - 965. 7. 56. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-1942534972&partnerID=MN8TOARS . 10.1016/j.addr.2003.10.038 . Advanced Drug Delivery Reviews

Improving lipoplex-mediated gene transfer into C6 glioma cells and primary neurons

Girão Da Cruz, M.T.; Simões, S.; Pedroso De Lima, M.C., 2004. Experimental Neurology. 65 - 75. 1. 187. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-1842764711&partnerID=MN8TOARS . 10.1016/j.expneurol.2003.12.013 . Experimental Neurology

Two African swine fever virus proteins derived from a common precursor exhibit different nucleocytoplasmic transport activities

Eulálio, A.; Nunes-Correia, I.; Carvalho, A.L.; Faro, C.; Citovsky, V.; Simões, S.; De Lima, M.C.P., 2004. Journal of Virology. 9731 - 9739. 18. 78. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-4444281591&partnerID=MN8TOARS . 10.1128/JVI.78.18.9731-9739.2004 . Journal of Virology

Kinetics of influenza virus fusion with the endosomal and plasma membranes of cultured cells. Effect of temperature

Nunes-Correia, I.; Nir, S.; Pedroso De Lima, M.C., 2003. Journal of Membrane Biology. 21 - 26. 1. 195. 2003. http://www.scopus.com/inward/record.url?eid=2-s2.0-0141540481&partnerID=MN8TOARS . 10.1007/s00232-003-2040-x . Journal of Membrane Biology

Cationic liposomes for gene delivery: From biophysics to biological applications

Pedroso de Lima, M.C.; Neves, S.; Filipe, A.; Düzgünes, N.; Simões, S., 2003. Current Medicinal Chemistry. 1221 - 1231. 14. 10. 2003. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037805617&partnerID=MN8TOARS . Current Medicinal Chemistry

Cationic liposomes for gene delivery: Novel cationic lipids and enhancement by proteins and peptides

Düzgünes, N.; Tros de Ilarduya, C.; Simões, S.; Zhdanov, R.I.; Konopka, K.; Pedroso de Lima, M.C., 2003. Current Medicinal Chemistry. 1213 - 1220. 14. 10. 2003. http://www.scopus.com/inward/record.url?eid=2-s2.0-18744426403&partnerID=MN8TOARS . Current Medicinal Chemistry

Cytotoxic effects of metal complexes of biogenic polyamines. I. Platinum(II) spermidine compounds: Prediction of their antitumour activity

Marques, M.P.M.; Girão, T.; Pedroso De Lima, M.C.; Gameiro, A.; Pereira, E.; Garcia, P., 2002. Biochimica et Biophysica Acta - Molecular Cell Research. 63 - 70. 1. 1589. 2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037070098&partnerID=MN8TOARS . 10.1016/S0167-4889(01)00186-0 . Biochimica et Biophysica Acta - Molecular Cell Research

Protein-associated lipoplexes: Novel strategies to enhance gene delivery mediated by lipid-based particles

Simões, S.; Fonseca, C.; Faneca, H.; Düzgünes, N.; Pedroso de Lima, M.C., 2002. S.T.P. Pharma Sciences. 339 - 344. 6. 12. 2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036855226&partnerID=MN8TOARS . S.T.P. Pharma Sciences

Evaluation of lipid-based reagents to mediate intracellular gene delivery

Faneca, H.; Simões, S.; Pedroso de Lima, M.C., 2002. Biochimica et Biophysica Acta - Biomembranes. 23 - 33. SUPPL.. 1567. 2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037164699&partnerID=MN8TOARS . 10.1016/S0005-2736(02)00545-X . Biochimica et Biophysica Acta - Biomembranes

Fluorescent probes for monitoring virus fusion kinetics: Comparative evaluation of reliability

Nunes-Correia, I.; Eulálio, A.; Nir, S.; Düzgünes, N.; Ramalho-Santos, J.; Pedroso De Lima, M.C., 2002. Biochimica et Biophysica Acta - Biomembranes. 65 - 75. 1. 1561. 2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037133738&partnerID=MN8TOARS . 10.1016/S0005-2736(01)00457-6 . Biochimica et Biophysica Acta - Biomembranes

On the mechanisms of internalization and intracellular delivery mediated by pH-sensitive liposomes

Simões, Sérgio; Slepushkin, Vladimir; Düzgünes, Nejat; Pedroso de Lima, Maria C., 2001. Biochimica et Biophysica Acta (BBA) - Biomembranes. 23 - 37. 1. 1515. 2001. http://dx.doi.org/10.1016/s0005-2736(01)00389-3 . 10.1016/s0005-2736(01)00389-3 . Biochimica et Biophysica Acta (BBA) - Biomembranes

Enhanced inhibition of HIV-1 replication in macrophages by antisense oligonucleotides, ribozymes and acyclic nucleoside phosphonate analogs delivered in pH-sensitive liposomes

Düzgünes, N.; Simões, S.; Slepushkin, V.; Pretzer, E.; Rossi, J.J.; De Clercq, E.; Antao, V.P.; Collins, M.L.; Pedroso de Lima, M.C., 2001. Nucleosides, Nucleotides and Nucleic Acids. 515 - 523. 4-7. 20. 2001. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034844548&partnerID=MN8TOARS . 10.1081/NCN-100002327 . Nucleosides, Nucleotides and Nucleic Acids

Fusion and infection of influenza and sendai viruses as modulated by dextran sulfate: A comparative study

Ramalho-Santos, J.; Pedroso De Lima, M.C., 2001. Bioscience Reports. 293 - 304. 3. 21. 2001. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035721635&partnerID=MN8TOARS . 10.1023/A:1013282015121 . Bioscience Reports

Delivery of novel macromolecular drugs against HIV-1

Düzgünes, N.; Simões, S.; Konopka, K.; Rossi, J.J.; Pedroso De Lima, M.C., 2001. Expert Opinion on Biological Therapy. 949 - 970. 6. 1. 2001. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035514271&partnerID=MN8TOARS . 10.1517/14712598.1.6.949 . Expert Opinion on Biological Therapy

Cationic lipid-DNA complexes in gene delivery: From biophysics to biological applications

Pedroso De Lima, M.C.; Simões, S.; Pires, P.; Faneca, H.; Düzgünes, N., 2001. Advanced Drug Delivery Reviews. 277 - 294. 2-3. 47. 2001. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035946624&partnerID=MN8TOARS . 10.1016/S0169-409X(01)00110-7 . Advanced Drug Delivery Reviews

Kinetic analysis of the initial steps involved in lipoplex-cell interactions: Effect of various factors that influence transfection activity

Girão Da Cruz, M.Teresa; Simões, S.; Pires, P.P.C.; Nir, S.; Pedroso De Lima, M.C., 2001. Biochimica et Biophysica Acta - Biomembranes. 136 - 151. 1-2. 1510. 2001. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035830655&partnerID=MN8TOARS . 10.1016/S0005-2736(00)00342-4 . Biochimica et Biophysica Acta - Biomembranes

Human serum albumin enhances DNA transfection by lipoplexes and confers resistance to inhibition by serum

Simões, S.; Slepushkin, V.; Pires, P.; Gaspar, R.; Pedroso De Lima, M.C.; Düzgünes, N., 2000. Biochimica et Biophysica Acta - Biomembranes. 459 - 469. 2. 1463. 2000. http://www.scopus.com/inward/record.url?eid=2-s2.0-0342680042&partnerID=MN8TOARS . 10.1016/S0005-2736(99)00238-2 . Biochimica et Biophysica Acta - Biomembranes

The saposin-like domain of the plant aspartic proteinase precursor is a potent inducer of vesicle leakage

Egas, C.; Lavoura, N.; Resende, R.; Brito, R.M.M.; Pires, E.; Pedroso De Lima, M.C.; Faro, C., 2000. Journal of Biological Chemistry. 38190 - 38196. 49. 275. 2000. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034623993&partnerID=MN8TOARS . 10.1074/jbc.M006093200 . Journal of Biological Chemistry

Mechanisms of gene transfer mediated by lipoplexes associated with targeting ligands or pH-sensitive peptides

Simões, S.; Slepushkin, V.; Pires, P.; Gaspar, R.; Pedroso De Lima, M.C.; Düzgünes, N., 1999. Gene Therapy. 1798 - 1807. 11. 6. 1999. http://www.scopus.com/inward/record.url?eid=2-s2.0-0342265531&partnerID=MN8TOARS . 10.1038/sj.gt.3301015 . Gene Therapy

Interaction of cationic liposomes and their DNA complexes with monocytic leukemia cells

Pires, P.; Simões, S.; Nir, S.; Gaspar, R.; Düzgünes, N.; Pedroso De Lima, M.C., 1999. Biochimica et Biophysica Acta - Biomembranes. 71 - 84. 1. 1418. 1999. http://www.scopus.com/inward/record.url?eid=2-s2.0-0344334068&partnerID=MN8TOARS . 10.1016/S0005-2736(99)00023-1 . Biochimica et Biophysica Acta - Biomembranes

Gene delivery mediated by cationic liposomes: From biophysical aspects to enhancement of transfection

Pedroso De Lima, M.C.; Simões, S.; Pires, P.; Gaspar, R.; Slepushkin, V.; Düzgünes, N., 1999. Molecular Membrane Biology. 103 - 109. 1. 16. 1999. http://www.scopus.com/inward/record.url?eid=2-s2.0-0345059269&partnerID=MN8TOARS . Molecular Membrane Biology

Interactions of influenza virus with cultured cells: Detailed kinetic modeling of binding and endocytosis

Nunes-Correia, I.; Ramalho-Santos, J.; Nir, S.; Pedroso De Lima, M.C., 1999. Biochemistry. 1095 - 1101. 3. 38. 1999. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033579938&partnerID=MN8TOARS . 10.1021/bi9812524 . Biochemistry

Transfection of human macrophages by lipoplexes via the combined use of transferrin and pH-sensitive peptides

Simões, S.; Slepushkin, V.; Pretzer, E.; Dazin, P.; Gaspar, R.; Pedroso De Lima, M.C.; Düzgünes, N., 1999. Journal of Leukocyte Biology. 270 - 279. 2. 65. 1999. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033028724&partnerID=MN8TOARS . Journal of Leukocyte Biology

Role of a transbilayer pH gradient in the membrane fusion activity of the influenza virus hemagglutinin: Use of the R18 assay to monitor membrane merging

Ramalho-Santos, J.; Pedroso De Lima, M.C., 1999. Biological Procedures Online. 107 - 113. 3. 1. 1999. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750619807&partnerID=MN8TOARS . Biological Procedures Online

Successful transfection of lymphocytes by ternary lipoplexes

Simões, S.; Slepushkin, V.; Gaspar, R.; Pedroso De Lima, M.C.; Düzgünes, N., 1999. Bioscience Reports. 601 - 609. 6. 19. 1999. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033513298&partnerID=MN8TOARS . Bioscience Reports

Liposome-mediated delivery of antiviral agents to human immunodeficiency virus-infected cells

Düzgünes, N.; Pretzer, E.; Simões, S.; Slepushkin, V.; Konopka, K.; Flasher, D.; Pedroso De Lima, M.C., 1999. Molecular Membrane Biology. 111 - 118. 1. 16. 1999. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032899408&partnerID=MN8TOARS . Molecular Membrane Biology

Cationic liposomes as gene transfer vectors: Barriers to successful application in gene therapy

Simões, S.; Pires, P.; Düzgünes, N.; Pedroso De Lima, M.C., 1999. Current Opinion in Molecular Therapeutics. 147 - 157. 2. 1. 1999. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037856537&partnerID=MN8TOARS . Current Opinion in Molecular Therapeutics

Sendai virus fusion activity as modulated by target membrane components

Nunes-Correia, I.; Ramalho-Santos, J.; Pedroso De Lima, M.C., 1998. Bioscience Reports. 59 - 68. 2. 18. 1998. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031727161&partnerID=MN8TOARS . 10.1023/A:1020180109275 . Bioscience Reports

Cholesterol affects African swine fever virus infection

Bernardes, C.; António, A.; Pedroso De Lima, M.C.; Valdeira, M.L., 1998. Biochimica et Biophysica Acta - Lipids and Lipid Metabolism. 19 - 25. 1. 1393. 1998. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032584552&partnerID=MN8TOARS . 10.1016/S0005-2760(98)00051-4 . Biochimica et Biophysica Acta - Lipids and Lipid Metabolism

Gene delivery by negatively charged ternary complexes of DNA, cationic liposomes and transferrin or fusigenic peptides

Simões, S.; Slepushkin, V.; Caspar, R.; Pedroso de Lima, M.C.; Düzgünes, N., 1998. Gene Therapy. 955 - 964. 7. 5. 1998. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031825183&partnerID=MN8TOARS . Gene Therapy

The influenza virus hemagglutinin: A model protein in the study of membrane fusion

Ramalho-Santos, J.; De Lima, M.C.P., 1998. Biochimica et Biophysica Acta - Reviews on Biomembranes. 147 - 154. 1. 1376. 1998. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032578032&partnerID=MN8TOARS . 10.1016/S0304-4157(98)00002-1 . Biochimica et Biophysica Acta - Reviews on Biomembranes

Sterically stabilized pH-sensitive liposomes. Intracellular delivery of aqueous contents and prolonged circulation in vivo

Slepushkin, V.A.; Simões, S.; Dazin, P.; Newman, M.S.; Guo, L.S.; De Lima, M.C.P.; Düzgünes, N., 1997. Journal of Biological Chemistry. 2382 - 2388. 4. 272. 1997. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031032815&partnerID=MN8TOARS . 10.1074/jbc.272.4.2382 . published Journal of Biological Chemistry

Enhancement of cationic liposome-mediated gene delivery by transferrin and fusogenic peptides

Simoes, S.; Slepushkin, V.; Gaspar, R.; Pedroso de Lima, M.C.; Duzgunes, N., 1997. Proceedings of the Controlled Release Society. 659 - 660. 24. 1997. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030800943&partnerID=MN8TOARS . Proceedings of the Controlled Release Society

Evidence that synaptobrevin is involved in fusion between synaptic vesicles and synaptic plasma membrane vesicles

Almeida, M.T.; Ramalho-Santos, J.; Oliveira, C.R.; Pedroso De Lima, M.C., 1997. Biochemical and Biophysical Research Communications. 184 - 188. 1. 236. 1997. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031561140&partnerID=MN8TOARS . 10.1006/bbrc.1997.6928 . Biochemical and Biophysical Research Communications

Interaction of cationic liposomes with cells: Effect of several factors on liposome-cell fusion

Fires, P.; Simos, S.; Caspar, R.; Nir, S.; Dzgnes, N.; Lima, M.C., 1997. FASEB Journal. 9. 11. 1997. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750172630&partnerID=MN8TOARS . FASEB Journal

Fusion activity of the influenza virus hemagglutinin does not require a transbilayer pH gradient

Ramalho-Santos, J.; Pedroso De Lima, M.C., 1997. Biochimica et Biophysica Acta - Biomembranes. 194 - 198. 2. 1330. 1997. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030778514&partnerID=MN8TOARS . 10.1016/S0005-2736(97)00166-1 . Biochimica et Biophysica Acta - Biomembranes

The influenza virus hemagglutinin: a model protein in the study of membrane fusion

Ramalho-Santos, J.; Almeida, M.T.; De Pedroso Lima, M.C., 1997. FASEB Journal. 9. 11. 1997. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750094182&partnerID=MN8TOARS . FASEB Journal

Intracellular Ca<sup>2+</sup> homeostasis in human leukemic T-lymphocytes is affected by anticancer drugs: Implications for the regulation of leukemic cell growth

Da Silva, C.P.; De Oliveira, C.R.; De Lima, M.C.P., 1996. Journal of Experimental and Clinical Cancer Research. 129 - 138. 2. 15. 1996. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029816963&partnerID=MN8TOARS . Journal of Experimental and Clinical Cancer Research

Partial fusion activity of influenza virus toward liposomes and erythrocyte ghosts is distinct from viral inactivation

Ramalho-Santos, J.; De Pedroso Lima, M.C.; Nir, S., 1996. Journal of Biological Chemistry. 23902 - 23906. 39. 271. 1996. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029842067&partnerID=MN8TOARS . 10.1074/jbc.271.39.23902 . Journal of Biological Chemistry

Apoptosis as a mechanism of cell death induced by different chemotherapeutic drugs in human leukemic T-lymphocytes

Da Silva, C.P.; De Oliveira, C.R.; De Lima, M.D.C.P., 1996. Biochemical Pharmacology. 1331 - 1340. 10. 51. 1996. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029947269&partnerID=MN8TOARS . 10.1016/0006-2952(96)00041-X . Biochemical Pharmacology

Fusion activity of African swine fever virus towards target membranes: Lipid dependence and effect of dehydrating agents

Bernardes, C.; Valdeira, M.L.; Ramalho-Santos, J.; De Lima, M.C.P., 1995. Biochemistry and Molecular Biology International. 481 - 488. 3. 37. 1995. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029555561&partnerID=MN8TOARS . Biochemistry and Molecular Biology International

Effect of myosin phosphorylation on actomyosin ATPase activity: a flow microcalorimetric study

Aureliano, M.; Pedroso, M.C.; De Lima; Carvalho, A.P.; Pires, E.M.V., 1995. Thermochimica Acta. 59 - 66. C. 258. 1995. http://www.scopus.com/inward/record.url?eid=2-s2.0-4243321608&partnerID=MN8TOARS . 10.1016/0040-6031(94)02192-Q . Thermochimica Acta

Target cell membrane sialic acid modulates both binding and fusion activity of influenza virus

Pedroso de Lima, M.C.; Ramalho-Santos, J.; Flasher, D.; Slepushkin, V.A.; Nir, S.; Düzgunes, N., 1995. BBA - Biomembranes. 323 - 330. 2. 1236. 1995. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029026187&partnerID=MN8TOARS . 10.1016/0005-2736(95)00067-D . BBA - Biomembranes

Molecular mechanisms of chromium toxicity oxidation of hemoglobin

Alpoim, M.C.; Geraldes, C.F.; Oliveira, C.R.; Lima, M.C., 1995. Biochemical Society Transactions. 2. 23. 1995. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029010431&partnerID=MN8TOARS . Biochemical Society Transactions

The Use of Microcarrier Beads in Ion Transport NMR Studies of Perfused Cells

Zachariah, C.; Defreitas, D.M.; Castro, M.M.C.A.; Geraldes, C.F.G.C.; Lima, M.C.P.; Oliveira, C.R., 1995. Journal of Magnetic Resonance, Series B. 81 - 85. 1. 108. 1995. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029336712&partnerID=MN8TOARS . 10.1006/jmrb.1995.1106 . Journal of Magnetic Resonance, Series B

Fusion of Sendai virus with model membranes as affected by dehydrating agents and pH: Correlation between viral envelope hydrophobicity and fusion activity

Ramalho-Santos, J.; Negrao, R.; Da Conceicao Pedroso de Lima, M., 1994. Biochemistry and Molecular Biology International. 1121 - 1127. 6. 32. 1994. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028352917&partnerID=MN8TOARS . Biochemistry and Molecular Biology International

Role of hydrophobic interactions in the fusion activity of influenza and sendai viruses towards model membranes

Ramalho-Santos, J.; Negrão, R.; Lima, M.d.C.P.d., 1994. Bioscience Reports. 15 - 24. 1. 14. 1994. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028243606&partnerID=MN8TOARS . 10.1007/BF01901634 . Bioscience Reports

In vitro assay of the effects of anticancer drugs on leukemic T-cells by flow microcalorimetry

da Silva, C.P.; de Oliveira, C.R.; de Lima, M.d.C.P., 1994. International Journal of Pharmaceutics. 25 - 30. 1. 111. 1994. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028067072&partnerID=MN8TOARS . 10.1016/0378-5173(94)90398-0 . International Journal of Pharmaceutics

Parameters affecting fusion between liposomes and synaptosomes. Role of proteins, lipid peroxidation, pH and temperature

Almeida, M.T.; Ramalho-Santos, J.; Oliveira, C.R.; Pedroso de Lima, M.C., 1994. The Journal of Membrane Biology. 217 - 222. 2. 142. 1994. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027993101&partnerID=MN8TOARS . 10.1007/BF00234943 . The Journal of Membrane Biology

A common mechanism for influenza virus fusion activity and inactivation

Ramalho-Santos, J.; Nir, S.; Düzgünes, N.; De Carvalho, A.P.; De Lima, M.D.C.P., 1993. Biochemistry. 2771 - 2779. 11. 32. 1993. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027417786&partnerID=MN8TOARS . Biochemistry

Partition of Dopamine Antagonists into Synthetic Lipid Bilayers: the Effect of Membrane Structure and Composition

SARMENTO, A.B.; DE LIMA, M.C.P.; OLIVEJRA, C.R., 1993. Journal of Pharmacy and Pharmacology. 601 - 605. 7. 45. 1993. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027313440&partnerID=MN8TOARS . 10.1111/j.2042-7158.1993.tb05660.x . Journal of Pharmacy and Pharmacology

Fusion activity and inactivation of influenza virus: Kinetics of low pH-induced fusion with cultured cells

Duzgunes, N.; Pedroso de Lima, M.C.; Stamatatos, L.; Flasher, D.; Alford, D.; Friend, D.S.; Nir, S., 1992. Journal of General Virology. 27 - 37. 1. 73. 1992. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026504652&partnerID=MN8TOARS . Journal of General Virology

Interaction of clathrin with large unilamellar phospholipid vesicles at neutral pH. Lipid dependence and protein penetration

Seppen, J.; Ramalho-Santos, J.; de Carvalho, A.P.; ter Beest, M.; Kok, J.W.; Pedroso de Lima, M.C.; Hoekstra, D., 1992. BBA - Biomembranes. 209 - 215. 1. 1106. 1992. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026587918&partnerID=MN8TOARS . 10.1016/0005-2736(92)90240-M . BBA - Biomembranes

Application of flow microcalorimetry to the study of dehydrofolate reductase activities in crude tissue homogenates

Pereira da Silva, C.M.; Pedroso de Lima, M.C., 1992. Journal of Thermal Analysis. 821 - 833. 4. 38. 1992. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249838370&partnerID=MN8TOARS . 10.1007/BF01979414 . Journal of Thermal Analysis

Prediction of the cytotoxic effects of methotrexate on human T-lymphoblastic leukemia cells

Brandao, F.O.; Silva, C.P.; De Lima, M.C.P.; Oliveira, C.R., 1992. Journal of Experimental and Clinical Cancer Research. 51 - 62. 1. 11. 1992. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026778561&partnerID=MN8TOARS . Journal of Experimental and Clinical Cancer Research

Kinetic modeling of Sendai virus fusion with PC-12 cells: Effect of pH and temperature on fusion and viral inactivation

PEDROSO DE LIMA, M.d.C.; RAMALHO-SANTOS, J.; MARTINS, M.d.F.; PATO DE CARVALHO, A.; BAIROS, V.; NIR, S., 1992. European Journal of Biochemistry. 181 - 186. 1. 205. 1992. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026611884&partnerID=MN8TOARS . 10.1111/j.1432-1033.1992.tb16766.x . European Journal of Biochemistry

Fusion of Sendai virus with human HL-60 and CEM cells: different kinetics of fusion for two isolates

Pedroso de Lima, M.C.; Nir, S.; Flasher, D.; Klappe, K.; Hoekstra, D.; Düzgünes, N., 1991. BBA - Biomembranes. 446 - 454. 2. 1070. 1991. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025933996&partnerID=MN8TOARS . 10.1016/0005-2736(91)90085-M . BBA - Biomembranes

A flow microcalorimetric study of the inhibition of acetylcholinesterase by catecholamine derivatives

Silva, C.M.P.; De Lima, M.C.P.; Oliveira, C.R.; Carvlho, A.P., 1991. Thermochimica Acta. 221 - 230. C. 179. 1991. http://www.scopus.com/inward/record.url?eid=2-s2.0-0347785235&partnerID=MN8TOARS . 10.1016/0040-6031(91)80351-I . Thermochimica Acta

Interaction of antimycobacterial and anti-pneumocystis drugs with phospholipid membranes

Pedroso de Lima, M.C.; Chiche, B.H.; Debs, R.J.; Düzgünes, N., 1990. Chemistry and Physics of Lipids. 361 - 371. 4. 53. 1990. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025266029&partnerID=MN8TOARS . 10.1016/0009-3084(90)90034-O . Chemistry and Physics of Lipids

Fusion of enveloped viruses with cells and liposomes - Activity and inactivation

Nir, S.; Düzgünes, N.; Pedroso De Lima, M.C.; Hoekstra, D., 1990. Cell Biophysics. 181 - 201. 2. 17. 1990. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025641298&partnerID=MN8TOARS . 10.1007/BF02990496 . Cell Biophysics

Fusion of enveloped viruses with cells and liposomes

Shlomo Nir; Nejat Düzgünes; Maria C. Pedroso De Lima; Dick Hoekstra, 1990. Cell Biophysics. 181 - 201. 2. 17. 1990. https://doi.org/10.1007%2Fbf02990496 . 10.1007/bf02990496 . Cell Biophysics

Partition coefficients of dopamine antagonists in brain membranes and liposomes

Oliveira, C.R.; Lima, M.C.P.; Carvalho, C.A.M.; Leysen, J.E.; Carvalho, A.P., 1989. Biochemical Pharmacology. 2113 - 2120. 13. 38. 1989. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024340655&partnerID=MN8TOARS . 10.1016/0006-2952(89)90065-8 . Biochemical Pharmacology

Partition of ca<sup>2+</sup> antagonists in brain plasma membranes

Carvalho, C.M.; Oliveira, C.R.; Lima, M.P.; Leysen, J.E.; Carvalho, A.P., 1989. Biochemical Pharmacology. 2121 - 2127. 13. 38. 1989. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024475173&partnerID=MN8TOARS . 10.1016/0006-2952(89)90066-X . Biochemical Pharmacology

Cholesterol affects divalent cation-induced fusion and isothermal phase transitions of phospholipid membranes

Shavnin, S.A.; Pedroso de Lima, M.C.; Fedor, J.; Wood, P.; Bentz, J.; Düzgünes, N., 1988. BBA - Biomembranes. 405 - 416. 2. 946. 1988. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024262811&partnerID=MN8TOARS . 10.1016/0005-2736(88)90416-6 . BBA - Biomembranes

Microcalorimetric measurement of the enthalpies of transfer of a series of ortho- and para-alkoxyphenols from water to octan-1-ol and from isotonic solution to Escherichia coli cells

Beezer, A.E.; Lima, M.C.P.; Fox, G.G.; Arriaga, P.; Hunter, W.H.; Smith, B.V., 1987. Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases. 2705 - 2707. 9. 83. 1987. http://www.scopus.com/inward/record.url?eid=2-s2.0-0040404893&partnerID=MN8TOARS . 10.1039/F19878302705 . Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases

Quantitative structure-activity relationships: a group additivity scheme for biological response of E. coli to the action of o-, m- and p-alkoxyphenol

Beezer, A.E.; Gooch, C.A.; Hunter, W.H.; Lima, M.C.P.; Smith, B.V., 1987. International Journal of Pharmaceutics. 251 - 254. 1-3. 38. 1987. http://www.scopus.com/inward/record.url?eid=2-s2.0-0023628827&partnerID=MN8TOARS . 10.1016/0378-5173(87)90122-0 . International Journal of Pharmaceutics

Electrical conductivity, viscosity, and density of a two-component ionic system at its critical point

Schreiber, D.R.; De Lima, M.C.P.; Pitzer, K.S., 1987. Journal of Physical Chemistry. 4087 - 4091. 15. 91. 1987. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001151523&partnerID=MN8TOARS . Journal of Physical Chemistry

Solution thermodynamics for o-alkoxyphenols in water and in water-alcohol systems

Beezer, A.E.; Lima, M.C.P.; Fox, G.G.; Hunter, W.H.; Smith, B.V., 1987. Thermochimica Acta. 329 - 335. C. 116. 1987. http://www.scopus.com/inward/record.url?eid=2-s2.0-39349096788&partnerID=MN8TOARS . 10.1016/0040-6031(87)88193-5 . Thermochimica Acta

Solution thermodynamics for alkoxy phenols in alcohol and in water-alcohol systems

Beezer, A.E.; Volpe, P.L.O.; Lima, M.C.P.; Hunter, W.H., 1986. Journal of Solution Chemistry. 341 - 348. 4. 15. 1986. http://www.scopus.com/inward/record.url?eid=2-s2.0-34250123191&partnerID=MN8TOARS . 10.1007/BF00648888 . Journal of Solution Chemistry

Critical point and phase separation for an ionic system

Pitzer, K.S.; De Lima, M.C.P.; Schreiber, D.R., 1985. Journal of Physical Chemistry. 1854 - 1855. 10. 89. 1985. http://www.scopus.com/inward/record.url?eid=2-s2.0-0039672088&partnerID=MN8TOARS . Journal of Physical Chemistry

Thermodynamics of saturated electrolyte mixtures of NaCl with Na<inf>2</inf>SO<inf>4</inf> and with MgCl<inf>2</inf>

de Lima, M.C.P.; Pitzer, K.S., 1983. Journal of Solution Chemistry. 187 - 199. 3. 12. 1983. http://www.scopus.com/inward/record.url?eid=2-s2.0-33847144669&partnerID=MN8TOARS . 10.1007/BF00648056 . Journal of Solution Chemistry

Thermodynamics of saturated aqueous solutions including mixtures of NaCl, KCl, and CsCl

de Lima, M.C.P.; Pitzer, K.S., 1983. Journal of Solution Chemistry. 171 - 185. 3. 12. 1983. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001071681&partnerID=MN8TOARS . 10.1007/BF00648055 . Journal of Solution Chemistry

An investigation of non-faradaic electrocatalysis

Freund, P.L.; Conceiçâo, M.; Lima, P.; Spiro, M., 1982. Journal of Electroanalytical Chemistry. 241 - 252. 2. 133. 1982. http://www.scopus.com/inward/record.url?eid=2-s2.0-49049131731&partnerID=MN8TOARS . 10.1016/0368-1874(82)85141-1 . Journal of Electroanalytical Chemistry

A high-voltage constant-current generator

Traca, A.; Lima, M.C.P., 1980. Journal of Physics E: Scientific Instruments. 387 - 388. 4. 13. 1980. http://www.scopus.com/inward/record.url?eid=2-s2.0-0019008887&partnerID=MN8TOARS . 10.1088/0022-3735/13/4/006 . Journal of Physics E: Scientific Instruments

Transference numbers of a 2:2 electrolyte: Magnesium sulphate in water at 25°C

Lima, M.C.P.; Kumarasinghe, S.; Spiro, M., 1978. Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases. 1036 - 1044. 74. 1978. http://www.scopus.com/inward/record.url?eid=2-s2.0-37049106705&partnerID=MN8TOARS . 10.1039/F19787401036 . Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases

Analysis of organic amines and acids for water

Conceição, M.; Lima, P.; Spiro, M., 1976. Analytica Chimica Acta. 429 - 431. 2. 81. 1976. http://www.scopus.com/inward/record.url?eid=2-s2.0-49549135217&partnerID=MN8TOARS . 10.1016/S0003-2670(01)82042-0 . Analytica Chimica Acta

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