Innovative Therapies
RNA & Infection
Host-pathogen interactions
Bacterial Pathogens
Non-coding RNAs
MicroRNAs
High-throughput/high-content Screening
Host-pathogen interactions
Bacterial Pathogens
Non-coding RNAs
MicroRNAs
High-throughput/high-content Screening
Although the vast majority of bacteria are innocuous or even beneficial to mammalian hosts, infections by pathogenic bacteria account for a variety of diseases that, despite the existing antibiotics, continue to threaten public health worldwide.
Bacterial infections are characterized by a complex interplay between pathogens and host cells, leading to alterations of multiple host cell functions. However, the impact of bacterial infection on host RNA metabolism, including non-coding RNA (ncRNA) expression and function, as well as the reciprocal impact of host RNA metabolism in the life cycle of bacterial pathogens remain largely unexplored. A proper RNA metabolism is essential to a number host cell functions and therefore it is not surprising that it contributes to a proper defense against infection, and that, on the other hand, pathogens have evolved sophisticated mechanisms to subvert these pathways to their own benefit.
The main goal of the laboratory is to identify host ncRNAs (specifically, microRNAs and long ncRNAs) that regulate and/or are regulated during infection by human bacterial pathogens (e.g. Salmonella Typhimurium, Shigella flexneri, Campylobacter jejuni and Staphylococcus aureus) and to characterize the molecular mechanisms underlying their function. To achieve this goal we are applying systematic approaches, including gain- and loss-of-function screenings using genome-wide microRNA libraries and ncRNA profiling by RNA-seq.
The identification of these ncRNAs will be instrumental to the development of novel therapeutic approaches against pathogenic bacteria, either through the modulation of selected ncRNAs, or of their targets.
Information about journal articles, updated at 18-04-2024, from platform CIÊNCIAVITAE.
Microscopy-based phenotypic profiling of infection by Staphylococcus aureus clinical isolates reveals intracellular lifestyle as a prevalent feature
Rodrigues Lopes, Inês; Laura Maria Alcantara; Ricardo Silva; Jérôme JOSSE; Elena Pedrero Vega; Ana Marina Cabrerizo; Melanie Bonhomme; et al, 2022. Nature Communications. 1. 13. 2022. 10.1038/s41467-022-34790-9 . Nature Communications
Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells
Carmen Aguilar; Susana Costa; Claire Maudet; R. P. Vivek-Ananth; Sara Zaldívar-López; Juan J. Garrido; Areejit Samal; Miguel Mano; Ana Eulalio, 2021. Nature Communications. 2021. 10.1038/s41467-021-23593-z . Nature Communications
Dysregulated endolysosomal trafficking in cells arrested in the G1 phase of the host cell cycle impairs Salmonella vacuolar replication
Lisowski, Clivia; Dias, Jane; Costa, Susana; Silva, Ricardo Jorge; Mano, Miguel; Eulalio, Ana, 2021. Autophagy. 1 - 16. 2021. 10.1080/15548627.2021.1999561 . published Autophagy
Functional screenings reveal different requirements for host microRNAs in Salmonella and Shigella infection
Aguilar, C.; Cruz, A.R.; Rodrigues Lopes, I.; Maudet, C.; Sunkavalli, U.; Silva, R.J.; Sharan, M.; et al, 2020. Nature Microbiology. 192 - 205. 1. 5. 2020. 10.1038/s41564-019-0614-3 . Nature Microbiology
Stress-induced host membrane remodeling protects from infection by non-motile bacterial pathogens
Tawk, C.; Nigro, G.; Rodrigues Lopes, I.; Aguilar, C.; Lisowski, C.; Mano, M.; Sansonetti, P.; Vogel, J.; Eulalio, A., 2018. The EMBO Journal. 23. 37. 2018. 10.15252/embj.201798529 . The EMBO Journal
Information about journal articles, updated at 18-04-2024, from platform CIÊNCIAVITAE.
Microscopy-based phenotypic profiling of infection by Staphylococcus aureus clinical isolates reveals intracellular lifestyle as a prevalent feature
Rodrigues Lopes, Inês; Laura Maria Alcantara; Ricardo Silva; Jérôme JOSSE; Elena Pedrero Vega; Ana Marina Cabrerizo; Melanie Bonhomme; et al, 2022. Nature Communications. 1. 13. 2022. 10.1038/s41467-022-34790-9 . Nature Communications
The induction of natural competence adapts staphylococcal metabolism to infection
Mar Cordero; Julia García-Fernández; Ivan C. Acosta; Ana Yepes; Jose Avendano-Ortiz; Clivia Lisowski; Babett Oesterreicht; et al, 2022. Nature Communications. 1. 13. 2022. 10.1038/s41467-022-29206-7 . Nature Communications
Dysregulated endolysosomal trafficking in cells arrested in the G1 phase of the host cell cycle impairs Salmonella vacuolar replication
Lisowski, Clivia; Dias, Jane; Costa, Susana; Silva, Ricardo Jorge; Mano, Miguel; Eulalio, Ana, 2021. Autophagy. 1 - 16. 2021. 10.1080/15548627.2021.1999561 . published Autophagy
Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells
Carmen Aguilar; Susana Costa; Claire Maudet; R. P. Vivek-Ananth; Sara Zaldívar-López; Juan J. Garrido; Areejit Samal; Miguel Mano; Ana Eulalio, 2021. Nature Communications. 2021. 10.1038/s41467-021-23593-z . Nature Communications
Functional screenings reveal different requirements for host microRNAs in Salmonella and Shigella infection
Aguilar, C.; Cruz, A.R.; Rodrigues Lopes, I.; Maudet, C.; Sunkavalli, U.; Silva, R.J.; Sharan, M.; et al, 2020. Nature Microbiology. 192 - 205. 1. 5. 2020. 10.1038/s41564-019-0614-3 . Nature Microbiology
MicroRNAs at the Host–Bacteria Interface: Host Defense or Bacterial Offense
Aguilar, C.; Mano, M.; Eulalio, A., 2019. Trends in Microbiology. 206 - 218. 3. 27. 2019. 10.1016/j.tim.2018.10.011 . Trends in Microbiology
Multifaceted roles of microRNAs in host-bacterial pathogen interaction
Aguilar, C.; Mano, M.; Eulalio, A., 2019. Microbiology Spectrum. 3. 7. 2019. 10.1128/microbiolspec.BAI-0002-2019 . Microbiology Spectrum
Shedding light on microRNA function via microscopy-based screening
Rodrigues Lopes, I.; Silva, R.J.; Caramelo, I.; Mano, M.; Eulalio, A., 2019. Methods. 2019. 10.1016/j.ymeth.2018.09.011 . Methods
Stress-induced host membrane remodeling protects from infection by non-motile bacterial pathogens
Tawk, C.; Nigro, G.; Rodrigues Lopes, I.; Aguilar, C.; Lisowski, C.; Mano, M.; Sansonetti, P.; Vogel, J.; Eulalio, A., 2018. The EMBO Journal. 23. 37. 2018. 10.15252/embj.201798529 . The EMBO Journal
Chlamydia preserves the mitochondrial network necessary for replication via microRNA-dependent inhibition of fission
Chowdhury, S.R.; Reimer, A.; Sharan, M.; Kozjak-Pavlovic, V.; Eulalio, A.; Prusty, B.K.; Fraunholz, M.; Karunakaran, K.; Rudel, T., 2017. Journal of Cell Biology. 1071 - 1089. 4. 216. 2017. 10.1083/jcb.201608063 . Journal of Cell Biology
APRICOT: An integrated computational pipeline for the sequence-based identification and characterization of RNA-binding proteins
Sharan, M.; Forstner, K.U.; Eulalio, A.; Vogel, J., 2017. Nucleic Acids Research. e96 - e96. 11. 45. 2017. 10.1093/nar/gkx137 . Nucleic Acids Research
A systematic analysis of the RNA-targeting potential of secreted bacterial effector proteins
Tawk, C.; Sharan, M.; Eulalio, A.; Vogel, J., 2017. Scientific Reports. 1. 7. 2017. 10.1038/s41598-017-09527-0 . Scientific Reports
Analysis of host microRNA function uncovers a role for miR-29b-2-5p in Shigella capture by filopodia
Sunkavalli, U.; Aguilar, C.; Silva, R.J.; Sharan, M.; Cruz, A.R.; Tawk, C.; Maudet, C.; Mano, M.; Eulalio, A., 2017. PLoS Pathogens. 4. 13. 2017. 10.1371/journal.ppat.1006327 . PLoS Pathogens
MicroRNA Screening and the Quest for Biologically Relevant Targets
Eulalio, A.; Mano, M., 2015. Journal of Biomolecular Screening. 1003 - 1017. 8. 20. 2015. 10.1177/1087057115578837 . Journal of Biomolecular Screening
Functional high-throughput screening identifies the miR-15 microRNA family as cellular restriction factors for Salmonella infection
Maudet, C.; Mano, M.; Sunkavalli, U.; Sharan, M.; Giacca, M.; Förstner, K.U.; Eulalio, A., 2014. Nature Communications. 5. 2014. 10.1038/ncomms5718 . Nature Communications
MicroRNAs in the interaction between host and bacterial pathogens
Maudet, C.; Mano, M.; Eulalio, A., 2014. FEBS Letters. 4140 - 4147. 22. 588. 2014. 10.1016/j.febslet.2014.08.002 . FEBS Letters
The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets
Huntzinger, E.; Kuzuoglu-Öztürk, D.; Braun, J.E.; Eulalio, A.; Wohlbold, L.; Izaurralde, E., 2013. Nucleic Acids Research. 978 - 994. 2. 41. 2013. 10.1093/nar/gks1078 . Nucleic Acids Research
The mammalian microRNA response to bacterial infections
Eulalio, A.; Schulte, L.N.; Voge, J., 2012. RNA Biology. 742 - 750. 6. 9. 2012. 10.4161/rna.20018 . RNA Biology
Functional screening identifies miRNAs inducing cardiac regeneration
Eulalio, A.; Mano, M.; Ferro, M.D.; Zentilin, L.; Sinagra, G.; Zacchigna, S.; Giacca, M., 2012. Nature. 376 - 381. 7429. 492. 2012. 10.1038/nature11739 . Nature
Terminal differentiation of cardiac and skeletal myocytes induces permissivity to AAV transduction by relieving inhibition imposed by DNA damage response proteins
Lovric, J.; Mano, M.; Zentilin, L.; Eulalio, A.; Zacchigna, S.; Giacca, M., 2012. Molecular Therapy. 2087 - 2097. 11. 20. 2012. 10.1038/mt.2012.144 . Molecular Therapy
Analysis of the host microRNA response to Salmonella uncovers the control of major cytokines by the let-7 family
Schulte, L.N.; Eulalio, A.; Mollenkopf, H.-J.; Reinhardt, R.; Vogel, J., 2011. EMBO Journal. 1977 - 1989. 10. 30. 2011. 10.1038/emboj.2011.94 . EMBO Journal
A candidate approach implicates the secreted Salmonella effector protein SpvB in P-body disassembly
Eulalio, A.; Fröhlich, K.S.; Mano, M.; Giacca, M.; Vogel, J., 2011. PLoS ONE. 3. 6. 2011. 10.1371/journal.pone.0017296 . PLoS ONE
Analysis of a to I editing of miRNA in macrophages exposed to salmonella
Heale, B.S.E.; Eulalio, A.; Schulte, L.; Vogel, J.; O'Connell, M.A., 2010. RNA Biology. 621 - 627. 5. 7. 2010. 10.4161.rna.7.5.13269 . RNA Biology
Deadenylation is a widespread effect of miRNA regulation
Eulalio, A.; Huntzinger, E.; Nishihara, T.; Rehwinkel, J.; Fauser, M.; Izaurralde, E., 2009. RNA. 21 - 32. 1. 15. 2009. 10.1261/rna.1399509 . RNA
The GW182 protein family in animal cells: New insights into domains required for miRNA-mediated gene silencing
Eulalio, A.; Tritschler, F.; Izaurralde, E., 2009. RNA. 1433 - 1442. 8. 15. 2009. 10.1261/rna.1703809 . RNA
A C-terminal silencing domain in GW182 is essential for miRNA function
Eulalio, A.; Helms, S.; Fritzsch, C.; Fauser, M.; Izaurralde, E., 2009. RNA. 1067 - 1077. 6. 15. 2009. 10.1261/rna.1605509 . RNA
The RRM domain in GW182 proteins contributes to miRNA-mediated gene silencing
Eulalio, A.; Tritschler, F.; Budie;ttner, R.; Weichenrieder, O.; Izaurralde, E.; Truffault, V., 2009. Nucleic Acids Research. 2974 - 2983. 9. 37. 2009. 10.1093/nar/gkp173 . Nucleic Acids Research
Structural Basis for the Mutually Exclusive Anchoring of P Body Components EDC3 and Tral to the DEAD Box Protein DDX6/Me31B
Tritschler, F.; Braun, J.E.; Eulalio, A.; Truffault, V.; Izaurralde, E.; Weichenrieder, O., 2009. Molecular Cell. 661 - 668. 5. 33. 2009. 10.1016/j.molcel.2009.02.014 . Molecular Cell
GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay
Eulalio, A.; Huntzinger, E.; Izaurralde, E., 2008. Nature Structural and Molecular Biology. 346 - 353. 4. 15. 2008. 10.1038/nsmb.1405 . Nature Structural and Molecular Biology
Getting to the Root of miRNA-Mediated Gene Silencing
Eulalio, A.; Huntzinger, E.; Izaurralde, E., 2008. Cell. 9 - 14. 1. 132. 2008. 10.1016/j.cell.2007.12.024 . Cell
Similar modes of interaction enable trailer hitch and EDC3 to associate with DCP1 and Me31B in distinct protein complexes
Tritschler, F.; Eulalio, A.; Helms, S.; Schmidt, S.; Coles, M.; Weichenrieder, O.; Izaurralde, E.; Truffault, V., 2008. Molecular and Cellular Biology. 6695 - 6708. 21. 28. 2008. 10.1128/MCB.00759-08 . Molecular and Cellular Biology
The C-terminal region of Ge-1 presents conserved structural features required for P-body localization
Jinek, M.; Eulalio, A.; Lingel, A.; Helms, S.; Conti, E.; Izaurralde, E., 2008. RNA. 1991 - 1998. 10. 14. 2008. 10.1261/rna.1222908 . RNA
African swine fever virus p10 protein exhibits nuclear import capacity and accumulates in the nucleus during viral infection
Nunes-Correia, I.; Rodríguez, J.M.; Eulálio, A.; Carvalho, A.L.; Citovsky, V.; Simões, S.; Faro, C.; Salas, M.L.; Pedroso de Lima, M.C., 2008. Veterinary Microbiology. 47 - 59. 1-2. 130. 2008. 10.1016/j.vetmic.2007.12.010 . Veterinary Microbiology
African swine fever virus p37 structural protein is localized in nuclear foci containing the viral DNA at early post-infection times
Eulálio, A.; Nunes-Correia, I.; Salas, J.; Salas, M.L.; Simões, S.; Pedroso de Lima, M.C., 2007. Virus Research. 18 - 27. 1-2. 130. 2007. 10.1016/j.virusres.2007.05.009 . Virus Research
A divergent Sm fold in EDC3 proteins mediates DCP1 binding and P-body targeting
Tritschler, F.; Eulalio, A.; Truffault, V.; Hartmann, M.D.; Helms, S.; Schmidt, S.; Coles, M.; Izaurralde, E.; Weichenrieder, O., 2007. Molecular and Cellular Biology. 8600 - 8611. 24. 27. 2007. 10.1128/MCB.01506-07 . Molecular and Cellular Biology
Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing
Eulalio, A.; Rehwinkel, J.; Stricker, M.; Huntzinger, E.; Yang, S.-F.; Doerks, T.; Dorner, S.; et al, 2007. Genes and Development. 2558 - 2570. 20. 21. 2007. 10.1101/gad.443107 . Genes and Development
Delving into the diversity of silencing pathways. Symposium on MicroRNAs and siRNAs: Biological Functions and Mechanisms
Dorner, S.; Eulalio, A.; Huntzinger, E.; Izaurralde, E., 2007. EMBO Reports. 723 - 729. 8. 8. 2007. 10.1038/sj.embor.7401015 . EMBO Reports
P bodies: At the crossroads of post-transcriptional pathways
Eulalio, A.; Behm-Ansmant, I.; Izaurralde, E., 2007. Nature Reviews Molecular Cell Biology. 9 - 22. 1. 8. 2007. 10.1038/nrm2080 . Nature Reviews Molecular Cell Biology
P-body formation is a consequence, not the cause, of RNA-mediated gene silencing
Eulalio, A.; Behm-Ansmant, I.; Schweizer, D.; Izaurralde, E., 2007. Molecular and Cellular Biology. 3970 - 3981. 11. 27. 2007. 10.1128/MCB.00128-07 . Molecular and Cellular Biology
Nuclear export of African swine fever virus p37 protein occurs through two distinct pathways and is mediated by three independent signals
Eulálio, A.; Nunes-Correia, I.; Carvalho, A.L.; Faro, C.; Citovsky, V.; Salas, J.; Salas, M.L.; Simões, S.; Pedroso De Lima, M.C., 2006. Journal of Virology. 1393 - 1404. 3. 80. 2006. 10.1128/JVI.80.3.1393-1404.2006 . Journal of Virology
Caveolae as an additional route for influenza virus endocytosis in MDCK cells
Nunes-Correia, I.; Eulálio, A.; Nir, S.; Pedroso De Lima, M.C., 2004. Cellular and Molecular Biology Letters. 47 - 60. 1. 9. 2004. Cellular and Molecular Biology Letters
Two African swine fever virus proteins derived from a common precursor exhibit different nucleocytoplasmic transport activities
Eulálio, A.; Nunes-Correia, I.; Carvalho, A.L.; Faro, C.; Citovsky, V.; Simões, S.; De Lima, M.C.P., 2004. Journal of Virology. 9731 - 9739. 18. 78. 2004. 10.1128/JVI.78.18.9731-9739.2004 . Journal of Virology
Fluorescent probes for monitoring virus fusion kinetics: Comparative evaluation of reliability
Nunes-Correia, I.; Eulálio, A.; Nir, S.; Düzgünes, N.; Ramalho-Santos, J.; Pedroso De Lima, M.C., 2002. Biochimica et Biophysica Acta - Biomembranes. 65 - 75. 1. 1561. 2002. 10.1016/S0005-2736(01)00457-6 . Biochimica et Biophysica Acta - Biomembranes
5 43 out of 43 Publications
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