Modulação metabólica
Nichos
Nanopartículas
Cancro
Terapia celular
Modulação metabólica de células estaminais cancerígenas
Modulação Biofísica do estado e destino celular
Modulação metabólica e imuno-terapia
O foco central do laboratório de RPN é a exploração das ligações entre metabolismo > transcrição > splicing alternativo > células estaminais/ doença, alicerçado nos princípios fundamentais da bioquímica e dando ênfase a translação e aplicação clínica na área das terapias celulares. Recentemente o laboratório demonstrou que a modulação bioquímica e física da estrutura da cromatina pode constituir uma ferramenta poderosa para alterar o processo de transcrição e expressão génica. Modulando um simples parâmetro bioquímico, a pressão osmótica extracelular, é possível alterar a estrutura da cromatina e a transcrição. Um estímulo Hypo-osmótico contribui para o relaxamento da cromatina e induz alterações na actividade da RNA polymerase II, constituindo uma opção para manipular a tomada de decisão em termos de estado celular. Esta tecnologia foi testada em protocolos de indução da pluripotência e de trans-diferenciação, em células que crescem em suspensão e/ ou agarradas a substratos, CD34+ MNCs do sangue do cordão umbilical, fibroblastos e células B, com acréscimo de eficiência. Alem disso, quando as células são cultivadas em substratos de baixa rigidez exibem um incremento de zonas de cromatina aberta e expressão de genes que facilitam a indução de pluripotência. O grupo é especialista na síntese e entrega de nanopartículas com diferentes cargas em células primárias e estaminais e patenteou uma NP ativável pela luz, capaz de libertar ácido retinóico dentro do nicho leucémico e induzir a diferenciação de células iniciadoras de leucemia. Neste contexto o grupo está a explorar a libertação de intermediários metabólicos capazes de modular nichos estaminais e microambientes tumorais no sentido de desenvolver novas terapias celulares para o cancro e medicina regenerativa.
Informação sobre artigos em revista, actualizada a 16-02-2025, a partir da plataforma CIÊNCIAVITAE.
Nanoparticle-encapsulated retinoic acid for the modulation of bone marrow hematopoietic stem cell niche
Emanuel Quartin; Susana Rosa; Sara Gonzalez-Anton; Laura Mosteo Lopez; Vitor Francisco; Delfim Duarte; Cristina Lo Celso; Ricardo Pires das Neves; Lino Ferreira, 2024. Bioactive Materials. 2024. https://doi.org/10.1016/j.bioactmat.2023.12.017 . 10.1016/j.bioactmat.2023.12.017 . Bioactive Materials
Reconstruction of cell-specific models capturing the influence of metabolism on DNA methylation in cancer
Tânia Barata; Vítor Pereira; Ricardo Pires das Neves; Miguel Rocha, 2024. Computers in Biology and Medicine. 2024. https://doi.org/10.1016/j.compbiomed.2024.108052 . 10.1016/j.compbiomed.2024.108052 . Computers in Biology and Medicine
Each Cellular Compartment Has a Characteristic Protein Reactive Cysteine Ratio Determining Its Sensitivity to Oxidation
Ricardo Pires das Neves; Mónica Chagoyen; Antonio Martinez-Lorente; Carlos Iñiguez; Ana Calatrava; Juana Calabuig; Francisco J. Iborra, 2023. Antioxidants. 2023. https://doi.org/10.3390/antiox12061274 . 10.3390/antiox12061274 . Antioxidants
Reconstruction of tissue-specific genome-scale metabolic models for human cancer stem cells
Barata, Tânia; Vieira, Vítor; Rodrigues, Rúben; Neves, Ricardo Pires das; Rocha, Miguel, 2022. Computers in Biology and Medicine. 142. 2022. http://dx.doi.org/10.1016/j.compbiomed.2021.105177 . 10.1016/j.compbiomed.2021.105177 . Computers in Biology and Medicine
Toxicological Profile of Umbilical Cord Blood-Derived Small Extracellular Vesicles
Rodrigues, Silvia C.; Cardoso, Renato M. S.; Gomes, Claudia F.; Duarte, Filipe V.; Freire, Patricia C.; Neves, Ricardo; Simoes-Correia, Joana, 2021. Membranes. 9. 11. 2021. http://dx.doi.org/10.3390/membranes11090647 . 10.3390/membranes11090647 . Membranes
Immunomodulatory Properties of Umbilical Cord Blood-Derived Small Extracellular Vesicles and Their Therapeutic Potential for Inflammatory Skin Disorders
Sílvia C. Rodrigues; Renato M. S. Cardoso; Patricia C. Freire; Cláudia F. Gomes; Filipe V. Duarte; Ricardo Pires das Neves; Joana Simões-Correia, 2021. International Journal of Molecular Sciences. 2021. https://doi.org/10.3390/ijms22189797 . 10.3390/ijms22189797 . International Journal of Molecular Sciences
Screening for Colorectal Cancer Leading into a New Decade: The “Roaring ‘20s” for Epigenetic Biomarkers?
Almeida-Lousada, Hélder; Mestre, André; Ramalhete, Sara; Price, Aryeh J.; de Mello, Ramon Andrade; Marreiros, Ana D.; Neves, Ricardo Pires das; Castelo-Branco, Pedro, 2021. Current Oncology. 4874 - 4893. 6. 28. 2021. http://dx.doi.org/10.3390/curroncol28060411 . 10.3390/curroncol28060411 . Current Oncology
Identification of colorectal cancer associated biomarkers: an integrated analysis of miRNA expression
Fonseca, André; Ramalhete, Sara Ventura; Mestre, André; Pires das Neves, Ricardo; Marreiros, Ana; Castelo-Branco, Pedro; Roberto, Vânia Palma, 2021. Aging. 21991 - 22029. 18. 13. 2021. http://dx.doi.org/10.18632/aging.203556 . 10.18632/aging.203556 . Aging
Development of an optimized and scalable method for isolation of umbilical cord blood-derived small extracellular vesicles for future clinical use
Renato M. S. Cardoso; Silvia C. Rodrigues; Claudia F. Gomes; Filipe V. Duarte; Maryse Romao; Ermelindo C. Leal; Patricia C. Freire; Ricardo Neves; Joana Simões-Correia, 2021. STEM CELLS Translational Medicine. 2021. https://doi.org/10.1002/sctm.20-0376 . 10.1002/sctm.20-0376 . STEM CELLS Translational Medicine
Correction to: In vitro mechanistic studies on a-amanitin and its putative antidotes
Daniela Ferreira Rodrigues; Ricardo Pires das Neves; Alexandra T. P. Carvalho; Maria Lourdes Bastos; Vera M. Costa; Félix Carvalho, 2020. Archives of Toxicology. 2020. https://doi.org/10.1007/s00204-020-02735-0 . 10.1007/s00204-020-02735-0 . Archives of Toxicology
In vitro mechanistic studies on a-amanitin and its putative antidotes
Daniela Ferreira Rodrigues; Ricardo Pires das Neves; Alexandra T. P. Carvalho; Maria Lourdes Bastos; Vera M. Costa; Félix Carvalho, 2020. Archives of Toxicology. 2020. https://doi.org/10.1007/s00204-020-02718-1 . 10.1007/s00204-020-02718-1 . Archives of Toxicology
Soft culture substrates favor stem-like cellular phenotype and facilitate reprogramming of human mesenchymal stem/stromal cells (hMSCs) through mechanotransduction
Neves, Ricardo, 2019. Scientific Reports. 2019. http://dx.doi.org/10.1038/s41598-019-45352-3 . 10.1038/s41598-019-45352-3 . Scientific Reports
Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
Neves, Ricardo, 2018. Scientific Reports. 2018. http://dx.doi.org/10.1038/s41598-018-25517-2 . 10.1038/s41598-018-25517-2 . Scientific Reports
Mitochondrial levels determine variability in cell death by modulating apoptotic gene expression
Márquez-Jurado, S.; Díaz-Colunga, J.; Das Neves, R.P.; Martinez-Lorente, A.; Almazán, F.; Guantes, R.; Iborra, F.J., 2018. Nature Communications. 1. 9. 2018. http://www.scopus.com/inward/record.url?eid=2-s2.0-85041107950&partnerID=MN8TOARS . 10.1038/s41467-017-02787-4 . Nature Communications
Validating the RedMIT/GFP-LC3 Mouse Model by Studying Mitophagy in Autosomal Dominant Optic Atrophy Due to the OPA1Q285STOP Mutation
Neves, Ricardo, 2018. Frontiers in Cell and Developmental Biology. 2018. http://dx.doi.org/10.3389/fcell.2018.00103 . 10.3389/fcell.2018.00103 . Frontiers in Cell and Developmental Biology
Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation
Boto, C.; Quartin, E.; Cai, Y.; Martín-Lorenzo, A.; Cenador, M.B.G.; Pinto, S.; Gupta, R.; et al, 2017. Nature Communications. 8. 2017. http://www.scopus.com/inward/record.url?eid=2-s2.0-85026559803&partnerID=MN8TOARS . 10.1038/ncomms15204 . Nature Communications
Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes
Gouveia, P.J.; Rosa, S.; Ricotti, L.; Abecasis, B.; Almeida, H.V.; Monteiro, L.; Nunes, J.; et al, 2017. Biomaterials. 213 - 228. 139. 2017. http://www.scopus.com/inward/record.url?eid=2-s2.0-85020728965&partnerID=MN8TOARS . 10.1016/j.biomaterials.2017.05.048 . Biomaterials
Global variability in gene expression and alternative splicing is modulated by mitochondrial content
Guantes, R.; Rastrojo, A.; Neves, R.; Lima, A.; Aguado, B.; Iborra, F.J., 2015. Genome Research. 633 - 644. 5. 125. 2015. http://www.scopus.com/inward/record.url?eid=2-s2.0-84929660832&partnerID=MN8TOARS . 10.1101/gr.178426.114 . Genome Research
Boron nitride nanotube-mediated stimulation modulates F/G-actin ratio and mechanical properties of human dermal fibroblasts
Ricotti, L.; Das Neves, R.P.; Ciofani, G.; Canale, C.; Nitti, S.; Mattoli, V.; Mazzolai, B.; Ferreira, L.; Menciassi, A., 2014. Journal of Nanoparticle Research. 2. 16. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84894539117&partnerID=MN8TOARS . 10.1007/s11051-014-2247-z . Journal of Nanoparticle Research
Efficient pro-survival/angiogenic miRNA delivery by an MRI-detectable nanomaterial
Gomes, R.S.M.; Neves, R.P.D.; Cochlin, L.; Lima, A.; Carvalho, R.; Korpisalo, P.; Dragneva, G.; et al, 2013. ACS Nano. 3362 - 3372. 4. 7. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84876588539&partnerID=MN8TOARS . 10.1021/nn400171w . ACS Nano
Epigenetic engineering to reverse the Parkinson's expression state
Valente, A.X.C.N.; das Neves, R.P.; Oliveira, P.J., 2012. Parkinsonism and Related Disorders. 717 - 721. 6. 18. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84862191970&partnerID=MN8TOARS . 10.1016/j.parkreldis.2012.04.018 . Parkinsonism and Related Disorders
Mitochondrial variability as a source of extrinsic cellular noise
Johnston, I.G.; Gaal, B.; das Neves, R.P.; Enver, T.; Iborra, F.J.; Jones, N.S., 2012. PLoS Computational Biology. 3. 8. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84861161954&partnerID=MN8TOARS . 10.1371/journal.pcbi.1002416 . PLoS Computational Biology
Nanoparticles for intracellular-targeted drug delivery
Paulo, C.S.O.; Pires Das Neves, R.; Ferreira, L.S., 2011. Nanotechnology. 49. 22. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-81855212480&partnerID=MN8TOARS . 10.1088/0957-4484/22/49/494002 . Nanotechnology
a-MSH regulates intergenic splicing of MC1R and TUBB3 in human melanocytes
Dalziel, M.; Kolesnichenko, M.; Das Neves, R.P.; Iborra, F.; Goding, C.; Furger, A., 2011. Nucleic Acids Research. 2378 - 2392. 6. 39. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79953710861&partnerID=MN8TOARS . 10.1093/nar/gkq1125 . Nucleic Acids Research
Towards the maturation and characterization of smooth muscle cells derived from human embryonic stem cells
Vazão, H.; das Neves, R.P.; Grãos, M.; Ferreira, L., 2011. PLoS ONE. 3. 6. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79952523704&partnerID=MN8TOARS . 10.1371/journal.pone.0017771 . PLoS ONE
Connecting variability in global transcription rate to mitochondrial variability
das Neves, R.P.; Jones, N.S.; Andreu, L.; Gupta, R.; Enver, T.; Iborra, F.J., 2010. PLoS Biology. 12. 8. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-78650442826&partnerID=MN8TOARS . 10.1371/journal.pbio.1000560 . PLoS Biology
MicroRNA-210 regulates mitochondrial free radical response to hypoxia and krebs cycle in cancer cells by targeting iron sulfur cluster protein ISCU
Favaro, E.; Ramachandran, A.; McCormick, R.; Gee, H.; Blancher, C.; Crosby, M.; Devlin, C.; et al, 2010. PLoS ONE. 4. 5. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-77956402239&partnerID=MN8TOARS . 10.1371/journal.pone.0010345 . PLoS ONE
Association between active genes occurs at nuclear speckles and is modulated by chromatin environment
Brown, J.M.; Green, J.; Das Neves, R.P.; Neves, R.P.D.; Wallace, H.A.C.; Smith, A.J.H.; Hughes, J.; et al, 2008. Chemtracts. 411 - 413. 10. 21. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-66849113444&partnerID=MN8TOARS . 10.1083/jcb.200803174 . Chemtracts
Repeated administration of d-amphetamine results in a time-dependent and dose-independent sustained increase in urinary excretion of p-hydroxyamphetamine in mice
Carvalho, F.; Soares, M.E.; Fernandes, E.; Remião, F.; Carvalho, M.; Duarte, J.A.; Pires-das-Neves, R.; Pereira, M.D.L.; Bastos, M.D.L., 2007. Journal of Health Science. 371 - 377. 4. 53. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547566233&partnerID=MN8TOARS . 10.1248/jhs.53.371 . Journal of Health Science
Effect of Cr(V) on reproductive organ morphology and sperm parameters: An experimental study in mice
Pereira, M.D.L.; Das Neves, R.P.; Oliveira, H.; Santos, T.M.; De Jesus, J.P., 2005. Environmental Health: A Global Access Science Source. 4. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-23944463762&partnerID=MN8TOARS . 10.1186/1476-069X-4-9 . Environmental Health: A Global Access Science Source
Hypericum androsaemum infusion increases tert-butyl hydroperoxide-induced mice hepatotoxicity in vivo
Valentão, P.; Carvalho, M.; Carvalho, F.; Fernandes, E.; Neves, R.P.D.; Pereira, M.L.; Andrade, P.B.; Seabra, R.M.; Bastos, M.L., 2004. Journal of Ethnopharmacology. 345 - 351. 2-3. 94. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-4344716319&partnerID=MN8TOARS . 10.1016/j.jep.2004.06.012 . Journal of Ethnopharmacology
Protective activity of hesperidin and lipoic acid against sodium arsenite acute toxicity in mice
Pires Das Neves, R.N.; Carvalho, F.; Carvalho, M.; Fernandes, E.; Soares, E.; Bastos, M.D.L.; Pereira, M.D.L., 2004. Toxicologic Pathology. 527 - 535. 5. 32. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-4544289287&partnerID=MN8TOARS . 10.1080/01926230490502566 . Toxicologic Pathology
Cr(V) involvement in the toxicity pathway of testicular damage
Pereira, M.L.; Santos, T.M.; Pires das Neves, R.; Costa, F.G.; Pedrosa de Jesus, J., 2002. Asian Journal of Andrology. 153 - 155. 2. 4. 2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035987253&partnerID=MN8TOARS . Asian Journal of Andrology
Comparative histological studies on liver of mice exposed to Cr(VI) and Cr(V) compounds
Pires Das Neves, R.; Santos, T.M.; De Lourdes Pereira, M.; Pedrosa De Jesus, J., 2002. Human and Experimental Toxicology. 365 - 369. 7. 21. 2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036656032&partnerID=MN8TOARS . 10.1191/0960327102ht243oa . Human and Experimental Toxicology
Effect of 3,4-methylenedioxymethamphetamine ("ecstasy") on body temperature and liver antioxidant status in mice: Influence of ambient temperature
Carvalho, M.; Carvalho, F.; Remião, F.; De Lourdes Pereira, M.; Pires-Das-Neves, R.; De Lourdes Bastos, M., 2002. Archives of Toxicology. 166 - 172. 3. 76. 2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036214775&partnerID=MN8TOARS . 10.1007/s00204-002-0324-z . Archives of Toxicology
Chromium(VI) induced alterations in mouse spleen cells: A short-term assay
Das Neves, R.P.; Santos, T.M.; Pereira, M.D.L.; De Jesus, J.P., 2001. Cytobios. 27 - 34. 1 SUPPL.. 413. 2001. http://www.scopus.com/inward/record.url?eid=2-s2.0-0348238820&partnerID=MN8TOARS . Cytobios
5 36 de 36 Publicações
Nanomedicina e células troianas para intervenção no licho leucémico
2023-03-01
Este website utiliza cookies para melhorar a sua experiência.
