Interações Hospedeiro-patógeno
Patógenos Bacterianos
RNAs não Codificantes
Identificação e caracterização de microRNAs e lncRNAs que regulam e/ou são regulados durante a infecção por patógenos bacterianos
Estudo das interações hospedeiro-patógeno bacteriano usando tecnologias de high-throughput screening e RNA-seq
Embora a grande maioria das bactérias sejam inócua ou mesmo benéficas para os hospedeiros, as infecções por bactérias patogénicas são responsáveis por uma variedade de doenças que, apesar dos antibióticos existentes, continuam a ameaçar a saúde pública global.
As infecções bacterianas são caracterizadas por uma interacção complexa entre patógenos e células hospedeiras, levando a alterações de múltiplas funções das células hospedeiras. No entanto, o impacto da infecções bacterianas no metabolismo do RNA do hospedeiro, incluindo a expressão e função de RNAs não codificantes (ncRNA), bem como o impacto recíproco do metabolismo do RNA do hospedeiro no ciclo de vida dos patógenos bacterianos, permanecem em grande parte inexplorados. Um metabolismo de RNA adequado é essencial para várias funções da célula hospedeira e, consequentemente, não é surpreendente que contribua para uma defesa adequada contra a infecção e que, por outro lado, os patógenos tenham desenvolvido mecanismos sofisticados para subverter essas vias para seu próprio benefício.
O objetivo principal do laboratório é identificar ncRNAs do hospedeiro (especificamente, microRNAs e ncRNAs longos) que regulam e/ou são regulados durante a infecção por patógenos bacterianos humanos (por exemplo, Salmonella Typhimurium, Shigella flexneri, Campylobacter jejuni e Staphylococcus aureus) e caracterizar os mecanismos moleculares subjacentes à sua função.
Para alcançar este objetivo, são aplicadas abordagens sistemáticas, incluindo screenings de ganho e perda de função usando bibliotecas de microRNAs genome-wide e profiling de RNAs não codificantes por RNA-seq.
A identificação de ncRNAs será instrumental para o desenvolvimento de novas abordagens terapêuticas contra bactérias patogénicas, quer através da modulação de ncRNAs, quer dos seus alvos.
Informação sobre artigos em revista, actualizada a 01-12-2024, a partir da plataforma CIÊNCIAVITAE.
Microscopy-based phenotypic profiling of infection by Staphylococcus aureus clinical isolates reveals intracellular lifestyle as a prevalent feature
Rodrigues Lopes, Inês; Laura Maria Alcantara; Ricardo Silva; Jérôme JOSSE; Elena Pedrero Vega; Ana Marina Cabrerizo; Melanie Bonhomme; et al, 2022. Nature Communications. 1. 13. 2022. 10.1038/s41467-022-34790-9 . Nature Communications
Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells
Carmen Aguilar; Susana Costa; Claire Maudet; R. P. Vivek-Ananth; Sara Zaldívar-López; Juan J. Garrido; Areejit Samal; Miguel Mano; Ana Eulalio, 2021. Nature Communications. 2021. 10.1038/s41467-021-23593-z . Nature Communications
Dysregulated endolysosomal trafficking in cells arrested in the G1 phase of the host cell cycle impairs Salmonella vacuolar replication
Lisowski, Clivia; Dias, Jane; Costa, Susana; Silva, Ricardo Jorge; Mano, Miguel; Eulalio, Ana, 2021. Autophagy. 1 - 16. 2021. 10.1080/15548627.2021.1999561 . published Autophagy
Functional screenings reveal different requirements for host microRNAs in Salmonella and Shigella infection
Aguilar, C.; Cruz, A.R.; Rodrigues Lopes, I.; Maudet, C.; Sunkavalli, U.; Silva, R.J.; Sharan, M.; et al, 2020. Nature Microbiology. 192 - 205. 1. 5. 2020. 10.1038/s41564-019-0614-3 . Nature Microbiology
Stress-induced host membrane remodeling protects from infection by non-motile bacterial pathogens
Tawk, C.; Nigro, G.; Rodrigues Lopes, I.; Aguilar, C.; Lisowski, C.; Mano, M.; Sansonetti, P.; Vogel, J.; Eulalio, A., 2018. The EMBO Journal. 23. 37. 2018. 10.15252/embj.201798529 . The EMBO Journal
Informação sobre artigos em revista, actualizada a 01-12-2024, a partir da plataforma CIÊNCIAVITAE.
Microscopy-based phenotypic profiling of infection by Staphylococcus aureus clinical isolates reveals intracellular lifestyle as a prevalent feature
Rodrigues Lopes, Inês; Laura Maria Alcantara; Ricardo Silva; Jérôme JOSSE; Elena Pedrero Vega; Ana Marina Cabrerizo; Melanie Bonhomme; et al, 2022. Nature Communications. 1. 13. 2022. 10.1038/s41467-022-34790-9 . Nature Communications
The induction of natural competence adapts staphylococcal metabolism to infection
Mar Cordero; Julia García-Fernández; Ivan C. Acosta; Ana Yepes; Jose Avendano-Ortiz; Clivia Lisowski; Babett Oesterreicht; et al, 2022. Nature Communications. 1. 13. 2022. 10.1038/s41467-022-29206-7 . Nature Communications
Dysregulated endolysosomal trafficking in cells arrested in the G1 phase of the host cell cycle impairs Salmonella vacuolar replication
Lisowski, Clivia; Dias, Jane; Costa, Susana; Silva, Ricardo Jorge; Mano, Miguel; Eulalio, Ana, 2021. Autophagy. 1 - 16. 2021. 10.1080/15548627.2021.1999561 . published Autophagy
Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells
Carmen Aguilar; Susana Costa; Claire Maudet; R. P. Vivek-Ananth; Sara Zaldívar-López; Juan J. Garrido; Areejit Samal; Miguel Mano; Ana Eulalio, 2021. Nature Communications. 2021. 10.1038/s41467-021-23593-z . Nature Communications
Functional screenings reveal different requirements for host microRNAs in Salmonella and Shigella infection
Aguilar, C.; Cruz, A.R.; Rodrigues Lopes, I.; Maudet, C.; Sunkavalli, U.; Silva, R.J.; Sharan, M.; et al, 2020. Nature Microbiology. 192 - 205. 1. 5. 2020. 10.1038/s41564-019-0614-3 . Nature Microbiology
MicroRNAs at the Host–Bacteria Interface: Host Defense or Bacterial Offense
Aguilar, C.; Mano, M.; Eulalio, A., 2019. Trends in Microbiology. 206 - 218. 3. 27. 2019. 10.1016/j.tim.2018.10.011 . Trends in Microbiology
Multifaceted roles of microRNAs in host-bacterial pathogen interaction
Aguilar, C.; Mano, M.; Eulalio, A., 2019. Microbiology Spectrum. 3. 7. 2019. 10.1128/microbiolspec.BAI-0002-2019 . Microbiology Spectrum
Shedding light on microRNA function via microscopy-based screening
Rodrigues Lopes, I.; Silva, R.J.; Caramelo, I.; Mano, M.; Eulalio, A., 2019. Methods. 2019. 10.1016/j.ymeth.2018.09.011 . Methods
Stress-induced host membrane remodeling protects from infection by non-motile bacterial pathogens
Tawk, C.; Nigro, G.; Rodrigues Lopes, I.; Aguilar, C.; Lisowski, C.; Mano, M.; Sansonetti, P.; Vogel, J.; Eulalio, A., 2018. The EMBO Journal. 23. 37. 2018. 10.15252/embj.201798529 . The EMBO Journal
Chlamydia preserves the mitochondrial network necessary for replication via microRNA-dependent inhibition of fission
Chowdhury, S.R.; Reimer, A.; Sharan, M.; Kozjak-Pavlovic, V.; Eulalio, A.; Prusty, B.K.; Fraunholz, M.; Karunakaran, K.; Rudel, T., 2017. Journal of Cell Biology. 1071 - 1089. 4. 216. 2017. 10.1083/jcb.201608063 . Journal of Cell Biology
APRICOT: An integrated computational pipeline for the sequence-based identification and characterization of RNA-binding proteins
Sharan, M.; Forstner, K.U.; Eulalio, A.; Vogel, J., 2017. Nucleic Acids Research. e96 - e96. 11. 45. 2017. 10.1093/nar/gkx137 . Nucleic Acids Research
A systematic analysis of the RNA-targeting potential of secreted bacterial effector proteins
Tawk, C.; Sharan, M.; Eulalio, A.; Vogel, J., 2017. Scientific Reports. 1. 7. 2017. 10.1038/s41598-017-09527-0 . Scientific Reports
Analysis of host microRNA function uncovers a role for miR-29b-2-5p in Shigella capture by filopodia
Sunkavalli, U.; Aguilar, C.; Silva, R.J.; Sharan, M.; Cruz, A.R.; Tawk, C.; Maudet, C.; Mano, M.; Eulalio, A., 2017. PLoS Pathogens. 4. 13. 2017. 10.1371/journal.ppat.1006327 . PLoS Pathogens
MicroRNA Screening and the Quest for Biologically Relevant Targets
Eulalio, A.; Mano, M., 2015. Journal of Biomolecular Screening. 1003 - 1017. 8. 20. 2015. 10.1177/1087057115578837 . Journal of Biomolecular Screening
Functional high-throughput screening identifies the miR-15 microRNA family as cellular restriction factors for Salmonella infection
Maudet, C.; Mano, M.; Sunkavalli, U.; Sharan, M.; Giacca, M.; Förstner, K.U.; Eulalio, A., 2014. Nature Communications. 5. 2014. 10.1038/ncomms5718 . Nature Communications
MicroRNAs in the interaction between host and bacterial pathogens
Maudet, C.; Mano, M.; Eulalio, A., 2014. FEBS Letters. 4140 - 4147. 22. 588. 2014. 10.1016/j.febslet.2014.08.002 . FEBS Letters
The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets
Huntzinger, E.; Kuzuoglu-Öztürk, D.; Braun, J.E.; Eulalio, A.; Wohlbold, L.; Izaurralde, E., 2013. Nucleic Acids Research. 978 - 994. 2. 41. 2013. 10.1093/nar/gks1078 . Nucleic Acids Research
The mammalian microRNA response to bacterial infections
Eulalio, A.; Schulte, L.N.; Voge, J., 2012. RNA Biology. 742 - 750. 6. 9. 2012. 10.4161/rna.20018 . RNA Biology
Functional screening identifies miRNAs inducing cardiac regeneration
Eulalio, A.; Mano, M.; Ferro, M.D.; Zentilin, L.; Sinagra, G.; Zacchigna, S.; Giacca, M., 2012. Nature. 376 - 381. 7429. 492. 2012. 10.1038/nature11739 . Nature
Terminal differentiation of cardiac and skeletal myocytes induces permissivity to AAV transduction by relieving inhibition imposed by DNA damage response proteins
Lovric, J.; Mano, M.; Zentilin, L.; Eulalio, A.; Zacchigna, S.; Giacca, M., 2012. Molecular Therapy. 2087 - 2097. 11. 20. 2012. 10.1038/mt.2012.144 . Molecular Therapy
Analysis of the host microRNA response to Salmonella uncovers the control of major cytokines by the let-7 family
Schulte, L.N.; Eulalio, A.; Mollenkopf, H.-J.; Reinhardt, R.; Vogel, J., 2011. EMBO Journal. 1977 - 1989. 10. 30. 2011. 10.1038/emboj.2011.94 . EMBO Journal
A candidate approach implicates the secreted Salmonella effector protein SpvB in P-body disassembly
Eulalio, A.; Fröhlich, K.S.; Mano, M.; Giacca, M.; Vogel, J., 2011. PLoS ONE. 3. 6. 2011. 10.1371/journal.pone.0017296 . PLoS ONE
Analysis of a to I editing of miRNA in macrophages exposed to salmonella
Heale, B.S.E.; Eulalio, A.; Schulte, L.; Vogel, J.; O'Connell, M.A., 2010. RNA Biology. 621 - 627. 5. 7. 2010. 10.4161.rna.7.5.13269 . RNA Biology
Deadenylation is a widespread effect of miRNA regulation
Eulalio, A.; Huntzinger, E.; Nishihara, T.; Rehwinkel, J.; Fauser, M.; Izaurralde, E., 2009. RNA. 21 - 32. 1. 15. 2009. 10.1261/rna.1399509 . RNA
The GW182 protein family in animal cells: New insights into domains required for miRNA-mediated gene silencing
Eulalio, A.; Tritschler, F.; Izaurralde, E., 2009. RNA. 1433 - 1442. 8. 15. 2009. 10.1261/rna.1703809 . RNA
A C-terminal silencing domain in GW182 is essential for miRNA function
Eulalio, A.; Helms, S.; Fritzsch, C.; Fauser, M.; Izaurralde, E., 2009. RNA. 1067 - 1077. 6. 15. 2009. 10.1261/rna.1605509 . RNA
The RRM domain in GW182 proteins contributes to miRNA-mediated gene silencing
Eulalio, A.; Tritschler, F.; Budie;ttner, R.; Weichenrieder, O.; Izaurralde, E.; Truffault, V., 2009. Nucleic Acids Research. 2974 - 2983. 9. 37. 2009. 10.1093/nar/gkp173 . Nucleic Acids Research
Structural Basis for the Mutually Exclusive Anchoring of P Body Components EDC3 and Tral to the DEAD Box Protein DDX6/Me31B
Tritschler, F.; Braun, J.E.; Eulalio, A.; Truffault, V.; Izaurralde, E.; Weichenrieder, O., 2009. Molecular Cell. 661 - 668. 5. 33. 2009. 10.1016/j.molcel.2009.02.014 . Molecular Cell
GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay
Eulalio, A.; Huntzinger, E.; Izaurralde, E., 2008. Nature Structural and Molecular Biology. 346 - 353. 4. 15. 2008. 10.1038/nsmb.1405 . Nature Structural and Molecular Biology
Getting to the Root of miRNA-Mediated Gene Silencing
Eulalio, A.; Huntzinger, E.; Izaurralde, E., 2008. Cell. 9 - 14. 1. 132. 2008. 10.1016/j.cell.2007.12.024 . Cell
Similar modes of interaction enable trailer hitch and EDC3 to associate with DCP1 and Me31B in distinct protein complexes
Tritschler, F.; Eulalio, A.; Helms, S.; Schmidt, S.; Coles, M.; Weichenrieder, O.; Izaurralde, E.; Truffault, V., 2008. Molecular and Cellular Biology. 6695 - 6708. 21. 28. 2008. 10.1128/MCB.00759-08 . Molecular and Cellular Biology
The C-terminal region of Ge-1 presents conserved structural features required for P-body localization
Jinek, M.; Eulalio, A.; Lingel, A.; Helms, S.; Conti, E.; Izaurralde, E., 2008. RNA. 1991 - 1998. 10. 14. 2008. 10.1261/rna.1222908 . RNA
African swine fever virus p10 protein exhibits nuclear import capacity and accumulates in the nucleus during viral infection
Nunes-Correia, I.; Rodríguez, J.M.; Eulálio, A.; Carvalho, A.L.; Citovsky, V.; Simões, S.; Faro, C.; Salas, M.L.; Pedroso de Lima, M.C., 2008. Veterinary Microbiology. 47 - 59. 1-2. 130. 2008. 10.1016/j.vetmic.2007.12.010 . Veterinary Microbiology
African swine fever virus p37 structural protein is localized in nuclear foci containing the viral DNA at early post-infection times
Eulálio, A.; Nunes-Correia, I.; Salas, J.; Salas, M.L.; Simões, S.; Pedroso de Lima, M.C., 2007. Virus Research. 18 - 27. 1-2. 130. 2007. 10.1016/j.virusres.2007.05.009 . Virus Research
A divergent Sm fold in EDC3 proteins mediates DCP1 binding and P-body targeting
Tritschler, F.; Eulalio, A.; Truffault, V.; Hartmann, M.D.; Helms, S.; Schmidt, S.; Coles, M.; Izaurralde, E.; Weichenrieder, O., 2007. Molecular and Cellular Biology. 8600 - 8611. 24. 27. 2007. 10.1128/MCB.01506-07 . Molecular and Cellular Biology
Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing
Eulalio, A.; Rehwinkel, J.; Stricker, M.; Huntzinger, E.; Yang, S.-F.; Doerks, T.; Dorner, S.; et al, 2007. Genes and Development. 2558 - 2570. 20. 21. 2007. 10.1101/gad.443107 . Genes and Development
Delving into the diversity of silencing pathways. Symposium on MicroRNAs and siRNAs: Biological Functions and Mechanisms
Dorner, S.; Eulalio, A.; Huntzinger, E.; Izaurralde, E., 2007. EMBO Reports. 723 - 729. 8. 8. 2007. 10.1038/sj.embor.7401015 . EMBO Reports
P bodies: At the crossroads of post-transcriptional pathways
Eulalio, A.; Behm-Ansmant, I.; Izaurralde, E., 2007. Nature Reviews Molecular Cell Biology. 9 - 22. 1. 8. 2007. 10.1038/nrm2080 . Nature Reviews Molecular Cell Biology
P-body formation is a consequence, not the cause, of RNA-mediated gene silencing
Eulalio, A.; Behm-Ansmant, I.; Schweizer, D.; Izaurralde, E., 2007. Molecular and Cellular Biology. 3970 - 3981. 11. 27. 2007. 10.1128/MCB.00128-07 . Molecular and Cellular Biology
Nuclear export of African swine fever virus p37 protein occurs through two distinct pathways and is mediated by three independent signals
Eulálio, A.; Nunes-Correia, I.; Carvalho, A.L.; Faro, C.; Citovsky, V.; Salas, J.; Salas, M.L.; Simões, S.; Pedroso De Lima, M.C., 2006. Journal of Virology. 1393 - 1404. 3. 80. 2006. 10.1128/JVI.80.3.1393-1404.2006 . Journal of Virology
Caveolae as an additional route for influenza virus endocytosis in MDCK cells
Nunes-Correia, I.; Eulálio, A.; Nir, S.; Pedroso De Lima, M.C., 2004. Cellular and Molecular Biology Letters. 47 - 60. 1. 9. 2004. Cellular and Molecular Biology Letters
Two African swine fever virus proteins derived from a common precursor exhibit different nucleocytoplasmic transport activities
Eulálio, A.; Nunes-Correia, I.; Carvalho, A.L.; Faro, C.; Citovsky, V.; Simões, S.; De Lima, M.C.P., 2004. Journal of Virology. 9731 - 9739. 18. 78. 2004. 10.1128/JVI.78.18.9731-9739.2004 . Journal of Virology
Fluorescent probes for monitoring virus fusion kinetics: Comparative evaluation of reliability
Nunes-Correia, I.; Eulálio, A.; Nir, S.; Düzgünes, N.; Ramalho-Santos, J.; Pedroso De Lima, M.C., 2002. Biochimica et Biophysica Acta - Biomembranes. 65 - 75. 1. 1561. 2002. 10.1016/S0005-2736(01)00457-6 . Biochimica et Biophysica Acta - Biomembranes
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