Biotechnology

ACT – Advanced Cell Technology

High-throughput screening

miRNAs

Small molecules

Extracellular Vehicles

Regenerative Medicine


Research lines

Native and Bioengineered Extracellular Vehicles for Regerenative Medicine

Nature-inspired drug delivery systems

High-throughput phenotypic screening for Regenerative Medicine

Overview

Inspired by the basic principles of Cell Biology, ACT aims to restore/regenerate damaged/worn out tissues. The group uses (i) high-throughput screening approaches and stem cell-based assays to identify small molecules and miRNAs capable of enhancing tissue function and/or regeneration and (ii) nature-inspired delivery systems for in vivo delivery of the selected molecules. We are particularly interested on improving cell survival upon in vivo transplantation and on the use of extracellular vehicles as therapeutic tools, both in their native form as well as after modulation with molecules of interest.

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Featured Articles

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Information about journal articles, updated at 26-06-2022, from platform CIÊNCIAVITAE.

Exogenous loading of miRNAs into small extracellular vesicles

Abreu, Ricardo C.; Ramos, Cristiana V.; Becher, Clarissa; Lino, Miguel; Jesus, Carlos; Costa Martins, Paula A.; Martins, Patrícia A. T.; et al, 2021. Journal of Extracellular Vesicles. 10. 10. 2021. http://dx.doi.org/10.1002/jev2.12111 . 10.1002/jev2.12111 . Journal of Extracellular Vesicles

Native and bioengineered extracellular vesicles for cardiovascular therapeutics

de Abreu, Ricardo Cerqueira; Fernandes, Hugo; da Costa Martins, Paula A.; Sahoo, Susmita; Emanueli, Costanza; Ferreira, Lino, 2020. Nature Reviews Cardiology. 685 - 697. 11. 17. 2020. http://dx.doi.org/10.1038/s41569-020-0389-5 . 10.1038/s41569-020-0389-5 . Nature Reviews Cardiology

High-throughput identification of small molecules that affect human embryonic vascular development.

Vazão H; Rosa S; Barata T; Costa R; Pitrez PR; Honório I; de Vries MR; et al, 2017. 2017. http://europepmc.org/abstract/med/28348206 . 10.1073/pnas.1617451114 .

Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells

Ottaviani, L.; Juni, R.P.; de Abreu, R.C.; Sansonetti, M.; Sampaio-Pinto, V.; Halkein, J.; Hegenbarth, J.C.; et al, 2022. Molecular Therapy. 2022. http://dx.doi.org/10.1016/j.ymthe.2022.03.002 . 10.1016/j.ymthe.2022.03.002 . Molecular Therapy

A small molecule approach to engineering vascularized tissue

Doorn, Joyce; Fernandes, Hugo A. M.; Le, Bach Q.; van de Peppel, Jeroen; van Leeuwen, Johannes P. T. M.; De Vries, Margreet R.; Aref, Zeen; et al, 2013. Biomaterials. 3053 - 3063. 12. 34. 2013. 10.1016/j.biomaterials.2012.12.037 . Biomaterials


Publications

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Information about journal articles, updated at 26-06-2022, from platform CIÊNCIAVITAE.

MicroRNA-124-3p-enriched small extracellular vesicles as a therapeutic approach for Parkinson’s disease

Esteves, Marta; Abreu, Ricardo; Fernandes, Hugo; Serra-Almeida, Catarina; Martins, Patrícia A.T.; Barão, Marta; Cristóvão, Ana Clara; et al, 2022. Molecular Therapy. 2022. http://dx.doi.org/10.1016/j.ymthe.2022.06.003 . 10.1016/j.ymthe.2022.06.003 . Molecular Therapy

Extracellular vesicles enriched with an endothelial cell pro-survival microRNA affects skin tissue regeneration

Fernandes, Hugo; Zonnari, Alessandra; Abreu, Ricardo; Aday, Sezin; Barão, Marta; Albino, Inês; Lino, Miguel; et al, 2022. Molecular Therapy - Nucleic Acids. 307 - 327. 28. 2022. http://dx.doi.org/10.1016/j.omtn.2022.03.018 . 10.1016/j.omtn.2022.03.018 . Molecular Therapy - Nucleic Acids

Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells

Ottaviani, L.; Juni, R.P.; de Abreu, R.C.; Sansonetti, M.; Sampaio-Pinto, V.; Halkein, J.; Hegenbarth, J.C.; et al, 2022. Molecular Therapy. 2022. http://dx.doi.org/10.1016/j.ymthe.2022.03.002 . 10.1016/j.ymthe.2022.03.002 . Molecular Therapy

High-throughput screening of nanoparticles in drug delivery

Inês Tomé; Vitor Francisco; Hugo Fernandes; Lino Ferreira, 2021. APL Bioengineering. 2021. https://doi.org/10.1063/5.0057204 . 10.1063/5.0057204 . APL Bioengineering

Exogenous loading of miRNAs into small extracellular vesicles

Abreu, Ricardo C.; Ramos, Cristiana V.; Becher, Clarissa; Lino, Miguel; Jesus, Carlos; Costa Martins, Paula A.; Martins, Patrícia A. T.; et al, 2021. Journal of Extracellular Vesicles. 10. 10. 2021. http://dx.doi.org/10.1002/jev2.12111 . 10.1002/jev2.12111 . Journal of Extracellular Vesicles

A high-throughput screening platform to identify nanocarriers for efficient delivery of RNA-based therapies

Francisco, Vitor; Rebelo, Catarina; Rodrigues, Artur Filipe; Blersch, Josephine; Fernandes, Hugo; Ferreira, Lino, 2020. Methods. 2020. http://dx.doi.org/10.1016/j.ymeth.2020.12.002 . 10.1016/j.ymeth.2020.12.002 . Methods

A SOX2 Reporter System Identifies Gastric Cancer Stem-Like Cells Sensitive to Monensin

Pádua, Diana; Barros, Rita; Amaral, Ana Luísa; Mesquita, Patrícia; Freire, Ana Filipa; Sousa, Mafalda; Maia, André Filipe; et al, 2020. Cancers. 2. 12. 2020. http://dx.doi.org/10.3390/cancers12020495 . 10.3390/cancers12020495 . Cancers

Native and bioengineered extracellular vesicles for cardiovascular therapeutics

de Abreu, Ricardo Cerqueira; Fernandes, Hugo; da Costa Martins, Paula A.; Sahoo, Susmita; Emanueli, Costanza; Ferreira, Lino, 2020. Nature Reviews Cardiology. 685 - 697. 11. 17. 2020. http://dx.doi.org/10.1038/s41569-020-0389-5 . 10.1038/s41569-020-0389-5 . Nature Reviews Cardiology

The role of ENPP1/PC-1 in osteoinduction by calcium phosphate ceramics

Othman, Ziryan; Fernandes, Hugo; Groot, Arjan J.; Luider, Theo M.; Alcinesio, Alessandro; Pereira, Daniel de Melo; Guttenplan, Alexander P.M.; Yuan, Huipin; Habibovic, Pamela, 2019. Biomaterials. 12 - 24. 210. 2019. http://dx.doi.org/10.1016/j.biomaterials.2019.04.021 . 10.1016/j.biomaterials.2019.04.021 . Biomaterials

From fiber curls to mesh waves: a platform for the fabrication of hierarchically structured nanofibers mimicking natural tissue formation

Honglin Chen; Danielle F. Baptista; Giuseppe Criscenti; João Crispim; Hugo Fernandes; Clemens van Blitterswijk; Roman Truckenmüller; Lorenzo Moroni, 2019. Nanoscale. 14312 - 14321. 30. 11. 2019. https://doi.org/10.1039/C8NR10108F . 10.1039/C8NR10108F . Nanoscale

A High-Throughput Screening Method to Identify Compounds Displaying Human Vascular Embryonic Toxicity

Rosa, Susana; Pitrez, Patrícia; Fernandes, Hugo; Ferreira, Lino, 2019. Current Protocols in Stem Cell Biology. 1. 50. 2019. http://dx.doi.org/10.1002/cpsc.93 . 10.1002/cpsc.93 . Current Protocols in Stem Cell Biology

Soft-molecular imprinted electrospun scaffolds to mimic specific biological tissues.

Criscenti G; De Maria C; Longoni A; van Blitterswijk CA; Fernandes HAM; Vozzi G; Moroni L, 2018. Biofabrication. 2018. http://europepmc.org/abstract/med/30024388 . 10.1088/1758-5090/aad48a . Biofabrication

Muscle-Secreted Factors Improve Anterior Cruciate Ligament Graft Healing: An In Vitro and In Vivo Analysis

Ghebes, Corina Adriana; Groen, Nathalie; Cheuk, Yau Chuk; Fu, Sai Chuen; Fernandes, Hugo Machado; Saris, Daniel B.F., 2018. Tissue Engineering Part A. 322 - 334. 3-4. 24. 2018. http://dx.doi.org/10.1089/ten.tea.2016.0546 . 10.1089/ten.tea.2016.0546 . Tissue Engineering Part A

Bioactive Tape With BMP-2 Binding Peptides Captures Endogenous Growth Factors and Accelerates Healing After Anterior Cruciate Ligament Reconstruction.

Crispim JF; Fu SC; Lee YW; Fernandes HAM; Jonkheijm P; Yung PSH; Saris DBF, 2018. The American journal of sports medicine. 2018. http://europepmc.org/abstract/med/30074814 . 10.1177/0363546518787507 . The American journal of sports medicine

High throughput screening of photocatalytic conversion of pharmaceutical contaminants in water

Romão, Joana; Barata, David; Ribeiro, Nelson; Habibovic, Pamela; Fernandes, Hugo; Mul, Guido, 2017. Environmental Pollution. 1199 - 1207. 220. 2017. http://dx.doi.org/10.1016/j.envpol.2016.11.015 . 10.1016/j.envpol.2016.11.015 . Environmental Pollution

Prolotherapy Induces an Inflammatory Response in Human Tenocytes In Vitro

Ekwueme, Emmanuel C.; Mohiuddin, Mahir; Yarborough, Jazmin A.; Brolinson, P. Gunnar; Docheva, Denitsa; Fernandes, Hugo A. M.; Freeman, Joseph W., 2017. Clinical Orthopaedics and Related Research®. 2117 - 2127. 8. 475. 2017. http://dx.doi.org/10.1007/s11999-017-5370-1 . 10.1007/s11999-017-5370-1 . Clinical Orthopaedics and Related Research®

High-throughput identification of small molecules that affect human embryonic vascular development.

Vazão H; Rosa S; Barata T; Costa R; Pitrez PR; Honório I; de Vries MR; et al, 2017. 2017. http://europepmc.org/abstract/med/28348206 . 10.1073/pnas.1617451114 .

TGF-ß1 activation in human hamstring cells through growth factor binding peptides on polycaprolactone surfaces

Crispim, J.; Fernandes, H.A.M.; Fu, S.C.; Lee, Y.W.; Jonkheijm, P.; Saris, D.B.F., 2017. Acta Biomaterialia. 165 - 178. 53. 2017. http://dx.doi.org/10.1016/j.actbio.2017.01.066 . 10.1016/j.actbio.2017.01.066 . Acta Biomaterialia

High-Throughput Screening Assay Identifies Small Molecules Capable of Modulating the BMP-2 and TGF-ß1 Signaling Pathway

Ghebes, Corina-Adriana; van Lente, Jéré; Post, Janine Nicole; Saris, Daniel B. F.; Fernandes, Hugo, 2016. SLAS DISCOVERY: Advancing Life Sciences R&D. 40 - 50. 1. 22. 2016. http://dx.doi.org/10.1177/1087057116669346 . 10.1177/1087057116669346 . SLAS DISCOVERY: Advancing Life Sciences R&D

Means of enhancing bone fracture healing: optimal cell source, isolation methods and acoustic stimulation.

Ghebes CA; Braham MV; Zeegers AV; Renard AJ; Fernandes H; Saris DB, 2016. 2016. http://europepmc.org/abstract/med/27955656 .

Spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo

Higuera, Gustavo A.; Fernandes, Hugo; Spitters, Tim W.G.M.; van de Peppel, Jeroen; Aufferman, Nils; Truckenmueller, Roman; Escalante, Maryana; et al, 2015. Biomaterials. 190 - 202. 61. 2015. http://dx.doi.org/10.1016/j.biomaterials.2015.05.017 . 10.1016/j.biomaterials.2015.05.017 . Biomaterials

High throughput screening assay for the identification of compounds enhancing collagenous extracellular matrix production by ATDC5 cells.

Le B; Fernandes H; Bouten C; Karperien M; van Blitterswijk C; de Boer J, 2015. 2015. http://europepmc.org/abstract/med/25604243 . 10.1089/ten.TEC.2014.0088 .

Cross-Talk Between Human Tenocytes and Bone Marrow Stromal Cells Potentiates Extracellular Matrix Remodeling In Vitro

Ekwueme, Emmanuel C.; Shah, Jay V.; Mohiuddin, Mahir; Ghebes, Corina A.; Crispim, João F.; Saris, Daniël B.F.; Fernandes, Hugo A.M.; Freeman, Joseph W., 2015. Journal of Cellular Biochemistry. 684 - 693. 3. 117. 2015. http://dx.doi.org/10.1002/jcb.25353 . 10.1002/jcb.25353 . Journal of Cellular Biochemistry

Anterior cruciate ligament- and hamstring tendon-derived cells: in vitro differential properties of cells involved in ACL reconstruction

Ghebes, Corina Adriana; Kelder, Cindy; Schot, Thomas; Renard, Auke J.; Pakvis, Dean F. M.; Fernandes, Hugo; Saris, Daniel B., 2015. Journal of Tissue Engineering and Regenerative Medicine. 1077 - 1088. 4. 11. 2015. http://dx.doi.org/10.1002/term.2009 . 10.1002/term.2009 . Journal of Tissue Engineering and Regenerative Medicine

A small molecule approach to engineering vascularized tissue

Doorn, Joyce; Fernandes, Hugo A. M.; Le, Bach Q.; van de Peppel, Jeroen; van Leeuwen, Johannes P. T. M.; De Vries, Margreet R.; Aref, Zeen; et al, 2013. Biomaterials. 3053 - 3063. 12. 34. 2013. 10.1016/j.biomaterials.2012.12.037 . Biomaterials

Mesenchymal stromal cell-derived extracellular matrix influences gene expression of chondrocytes

Thakkar, Shraddha; Ghebes, Corina A.; Ahmed, Maqsood; Kelder, Cindy; van Blitterswijk, Clemens A.; Saris, Daniel; Fernandes, Hugo A. M.; Moroni, Lorenzo, 2013. Biofabrication. 2. 5. 2013. 10.1088/1758-5082/5/2/025003 . Biofabrication

Molecular mechanisms of biomaterial-driven osteogenic differentiation in human mesenchymal stromal cells

Barradas, Ana M. C.; Monticone, Veronica; Hulsman, Marc; Danoux, Charlene; Fernandes, Hugo; Birgani, Zeinab Tahmasebi; Barrere-de Groot, Florence; et al, 2013. Integrative Biology. 920 - 931. 7. 5. 2013. 10.1039/c3ib40027a . Integrative Biology

Endothelial Differentiation of Mesenchymal Stromal Cells

Portalska, Karolina Janeczek; Leferink, Anne; Groen, Nathalie; Fernandes, Hugo; Moroni, Lorenzo; van Blitterswijk, Clemens; de Boer, Jan, 2012. Plos One. 10. 7. 2012. 10.1371/journal.pone.0046842 . Plos One

The influence of genetic factors on the osteoinductive potential of calcium phosphate ceramics in mice

Barradas, Ana M.C.; Yuan, Huipin; van der Stok, Johan; Le Quang, Bach; Fernandes, Hugo; Chaterjea, Anindita; Hogenes, Marieke C.H.; et al, 2012. Biomaterials. 5696 - 5705. 23. 33. 2012. http://dx.doi.org/10.1016/j.biomaterials.2012.04.021 . 10.1016/j.biomaterials.2012.04.021 . Biomaterials

A calcium-induced signaling cascade leading to osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells

Barradas, Ana M.C.; Fernandes, Hugo A.M.; Groen, Nathalie; Chai, Yoke Chin; Schrooten, Jan; van de Peppel, Jeroen; van Leeuwen, Johannes P.T.M.; van Blitterswijk, Clemens A.; de Boer, Jan, 2012. Biomaterials. 3205 - 3215. 11. 33. 2012. http://dx.doi.org/10.1016/j.biomaterials.2012.01.020 . 10.1016/j.biomaterials.2012.01.020 . Biomaterials

Spatial distribution and survival of human and goat mesenchymal stromal cells on hydroxyapatite and ß-tricalcium phosphate.

Prins HJ; Fernandes H; Rozemuller H; van Blitterswijk C; de Boer J; Martens AC, 2012. 2012. http://europepmc.org/abstract/med/23255230 . 10.1002/term.1681 .

'Smart' biomaterials and osteoinductivity

Yuan, Huipin; Fernandes, Hugo; Habibovic, Pamela; de Boer, Jan; Barradas, Ana M. C.; de Ruiter, Ad; Walsh, William R.; van Blitterswijk, Clemens A.; de Bruijn, Joost D., 2011. Nature Reviews Rheumatology. 1 - 1. 4. 7. 2011. 10.1038/nrrheum.2010.210-c1 . Nature Reviews Rheumatology

Fabrication of Bioactive Composite Scaffolds by Electrospinning for Bone Regeneration

Nandakumar, Anandkumar; Fernandes, Hugo; de Boer, Jan; Moroni, Lorenzo; Habibovic, Pamela; van Blitterswijk, Clemens A., 2010. Macromolecular Bioscience. 1365 - 1373. 11. 10. 2010. http://dx.doi.org/10.1002/mabi.201000145 . 10.1002/mabi.201000145 . Macromolecular Bioscience

Endogenous Collagen Influences Differentiation of Human Multipotent Mesenchymal Stromal Cells

Fernandes, Hugo; Mentink, Anouk; Bank, Ruud; Stoop, Reinout; van Blitterswijk, Clemens; de Boer, Jan, 2010. Tissue Engineering Part a. 1693 - 1702. 5. 16. 2010. 10.1089/ten.tea.2009.0341 . Tissue Engineering Part a

In Vitro and In Vivo Bioluminescent Imaging of Hypoxia in Tissue-Engineered Grafts

Liu, Jun; Barradas, Ana; Fernandes, Hugo; Janssen, Frank; Papenburg, Bernke; Stamatialis, Dimitrios; Martens, Anton; van Blitterswijk, Clemens; de Boer, Jan, 2010. Tissue Engineering Part C-Methods. 479 - 485. 3. 16. 2010. 10.1089/ten.tec.2009.0278 . Tissue Engineering Part C-Methods

In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

Teixeira, S.; Fernandes, H.; Leusink, A.; van Blitterswijk, C.; Ferraz, M. P.; Monteiro, F. J.; de Boer, J., 2010. Journal of Biomedical Materials Research Part a. 567 - 575. 2. 93A. 2010. 10.1002/jbm.a.32532 . Journal of Biomedical Materials Research Part a

Osteoinductive ceramics as a synthetic alternative to autologous bone grafting

Yuan, Huipin; Fernandes, Hugo; Habibovic, Pamela; de Boer, Jan; Barradas, Ana M. C.; de Ruiter, Ad; Walsh, William R.; van Blitterswijk, Clemens A.; de Bruijn, Joost D., 2010. Proceedings of the National Academy of Sciences of the United States of America. 13614 - 13619. 31. 107. 2010. 10.1073/pnas.1003600107 . Proceedings of the National Academy of Sciences of the United States of America

Effect of Chordin-Like 1 on MC3T3-E1 and Human Mesenchymal Stem Cells

Fernandes, Hugo; Dechering, Koen; van Someren, Eugene; Steeghs, Ilse; Apotheker, Marion; Mentink, Anouk; van Blitterswijk, Clemens; de Boer, Jan, 2010. Cells Tissues Organs. 443 - 452. 6. 191. 2010. 10.1159/000281825 . Cells Tissues Organs

The Role of Collagen Crosslinking in Differentiation of Human Mesenchymal Stem Cells and MC3T3-E1 Cells

Fernandes, Hugo; Dechering, Koen; Van Someren, Eugene; Steeghs, Ilse; Apotheker, Marion; Leusink, Anouk; Bank, Ruud; et al, 2009. Tissue Engineering Part A. 3857 - 3867. 12. 15. 2009. http://dx.doi.org/10.1089/ten.tea.2009.0011 . 10.1089/ten.tea.2009.0011 . Tissue Engineering Part A

Extracellular matrix and tissue engineering applications

Fernandes, Hugo; Moroni, Lorenzo; van Blitterswijk, Clemens; de Boer, Jan, 2009. Journal of Materials Chemistry. 5474 - 5484. 31. 19. 2009. 10.1039/b822177d . Journal of Materials Chemistry

Growth, Metabolism, and Growth Inhibitors of Mesenchymal Stem Cells

Schop, Deborah; Janssen, Frank W.; van Rijn, Linda D.S.; Fernandes, Hugo; Bloem, Rolf M.; de Bruijn, Joost D.; van Dijkhuizen-Radersma, Riemke, 2009. Tissue Engineering Part A. 1877 - 1886. 8. 15. 2009. http://dx.doi.org/10.1089/ten.tea.2008.0345 . 10.1089/ten.tea.2008.0345 . Tissue Engineering Part A

cAMP/PKA Signaling Inhibits Osteogenic Differentiation and Bone Formation in Rodent Models

Siddappa, Ramakrishnaiah; Mulder, Winfried; Steeghs, Ilse; van de Klundert, Christine; Fernandes, Hugo; Liu, Jun; Arends, Roel; van Blitterswijk, Clemens; de Boer, Jan, 2009. Tissue Engineering Part A. 2135 - 2143. 8. 15. 2009. http://dx.doi.org/10.1089/ten.tea.2008.0512 . 10.1089/ten.tea.2008.0512 . Tissue Engineering Part A

Mesenchymal stem cell proliferation and morphological analysis on interconnected macroporous structures

Teixeira, S.; Fernandes, H.M.; De Boer, J.; Ferraz, M.P.; Monteiro, F.J., 2007. Key Engineering Materials. 1129 - 1132. 330-332 II. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846317063&partnerID=MN8TOARS . Key Engineering Materials

The response of human mesenchymal stem cells to osteogenic signals and its impact on bone tissue engineering.

Siddappa, Ramakrishnaiah; Fernandes, Hugo; Liu, Jun; van Blitterswijk, Clemens; de Boer, Jan, 2007. Current stem cell research & therapy. 209 - 20. 3. 2. 2007. 10.2174/157488807781696267 . Current stem cell research & therapy

Awards

2011

Best paper award 2010-11 (Dutch Bone and Mineral Society; Nov 2011)

2010

Science factor (best scientific project) (University Twente; Mar 2010)

2010

Finalist UTBusiness (best business plan) (University Twente; Mar 2010)

2018

FCT Scientific Employment Stimulus award

CEECIND/01880/2018

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