Aim

Coordinators:
Miguel Mano mano@ci.uc.pt 

Lecturers:
Luca Braga (International Centre for Genetic Engineering and Biotechnology,
Italy)
Mauro Giacca (King’s College London, UK)
Neville Sanjana (New York University, USA)
Peter Horvath (Szeged Biological Research Centre, Hungary and Institute for
Molecular Medicine Finland, Finland)
Roderick Beijersbergen (The Netherlands Cancer Institute, The Netherlands)
Valentina Adami (University of Trento, Italy)
Ana Eulalio (CNC & DCV, University of Coimbra)
Laura Alcantara (CNC, University of Coimbra)
Miguel Mano (CNC & DCV, University of Coimbra)
Ricardo Silva (CNC, University of Coimbra)

Program

PROGRAMME

Monday (February 1st)
9.30-10.00 – Welcome and introduction to the course 
Miguel Mano

10.00-12.30 – Screening 101: from basic concepts to practical aspects of implementing large-scale functional genomics screenings
Miguel Mano

14.00-15.00 – Principles of automated image analysis & lab tour
Miguel Mano

15.00-17.30 – Hands-on session I: image analysis – Columbus
Miguel Mano 

Tuesday (February 2nd)
9.30-11.30 – High throughput screening in drug discovery
Valentina Adami (University of Trento, Italy)

11.45-12.45 – Small molecule screening: examples from an academic Core Facility
Valentina Adami (University of Trento, Italy)

14.00-17.30 – Hands-on session II: image analysis – Cellprofiler
Ricardo Silva

Wednesday (February 3rd)
9.30-10.30 – CRISPR/Cas9: precise genome editing and beyond

Miguel Mano

10.45-12.15 – Pooled CRISPR screening: Finding needles in the haystack
Roderick Beijersbergen (The Netherlands Cancer Institute, The Netherlands)

15.00-16.30 –  Functional selection of tissue protective factors using AAV vector screenings in mice
Mauro Giacca (King’s College London, UK)

Thursday (February 4th)
9.30-10.30 – Drug repurposing for COVID-19 using image-based high-throughput screening
Luca Braga (International Centre for Genetic Engineering and Biotechnology, Italy)

10.45-11.45 – Exploring high-content screening to dissect the role of microRNAs in bacterial infection
Ana Eulalio

12.00-13.00 – Translational research and drug discovery for neglected tropical diseases: strategies based on drug combinations and repurposing
Laura Alcantara 

14.00-15.30 – New frontiers in target and drug discovery using massively-parallel CRISPR screens
Neville Sanjana (New York University, USA)

16.00-17.30 – Life beyond the pixels: deep learning methods for single cell analysis
Peter Horvath (Szeged Biological Research Centre, Hungary & Institute for Molecular Medicine Finland, Finland)

Friday (February 5th)
14.00-17.00 – Student presentations and wrap-up